Paper Titles
Temporal Modeling of Dengue Fever: A Comprehensive Literature Review
p.15
Extraction and Screening of ß-Sitosterol Compounds from Chloroform Extracts Barks of Kelor Plant (Moringa oleifera Limn) as a Candidate of Cancer Treatment Compounds
p.22
Phytochemical Screening and Antimicrobial Activity from Sonneratia caseolaris Fruit Extract
p.28
Antioxidant Activity Test Ethanol Extract of Lontar Fruits (Borassus flabellifer L.) and Ginger Bud (Etlingera elatior (Jack.) R.M. Sm) Using Ferric Reducing Antioxidant Power Method
p.34
Antibacterial Activity of Secondary Metabolite Compounds in Ethylacetate Extract of Rumput Mutiara (Hedyotis corymbosa (L.) Lamk)
p.38
Usnic Acid Derivate from Usnea sp. and Bioactivity against Arthemia salina Leach
p.45
Effect of Boiled Water Parasite Kapok (Dendrophthoe pentandra) Leaves on Total Cholesterol Levels and Histopathology of Hepar of Hypercholesterolemic Mice
p.51
Novel DNA Barcode Sequence Discovery from Transcriptome of Acheta domesticus: A Partial Mitochondrial DNA
p.59
Collection of Mitochondrial tRNA Sequences and Anticodon Identification for Acheta domesticus
p.65

Antibacterial Activity of Secondary Metabolite Compounds in Ethylacetate Extract of Rumput Mutiara (Hedyotis corymbosa (L.) Lamk)

Article Preview

Abstract:

Plant Rumput Mutiara (Hedyotis corymbosa (L.) Lamk) is one of the family species of Rubiaceae which is used as a traditional medicine that is effective in healing boils, acne, anti-inflammatory, and anticancer. Research methods include maceration, fractionation, purification, class test and bioactivity test with Kirby-Bauer diffusion method using E.coli bacteria. The results of this research are pure isolates with white crystal needle shape with melting point 137-138°C. Pure isolates were analyzed using FTIR, and by the IR spectrum which showed the absorption band at wave numbers 3435.22 cm-1 indicated the presence of –OH, CH3 and-CH2- aliphatic groups (2956.87 cm-1; 2935.66 cm-1; 2893.22 cm-1; and 2866.22 cm-1), C = C (1641.42 cm-1), -CH3 and-CH2- bending (1462.04 cm-1 and 1377.17 cm- 1), CO (1056.99 cm-1), and = CH (964.41 cm-1). Based on the result, it showed that the isolate was steroid group which has low antibacterial activity againts E.coli with inhibition power of 10 mm.

You might also be interested in these eBooks
Green Materials and Technology View Preview

Info:

Periodical:

Materials Science Forum (Volume 967)

Pages:

38-44

DOI:

https://doi.org/10.4028/www.scientific.net/MSF.967.38

Citation:

Cite this paper

Online since:

August 2019

Authors:

Pince Salempa*, Muharram Muharram, Rika Fajri

Keywords:

Antibacterial, E. coli, Hedyotis corymbosa (L.) Lamk, Isolation, Steroid

Export:

RIS, BibTeX

Price:

Permissions CCC:

Request Permissions

Permissions PLS:

Request Permissions

Сopyright:

© 2019 Trans Tech Publications Ltd. All Rights Reserved

Share:

Citation:

* - Corresponding Author

References

[1] M.N. Alam, N.J. Bristi, M. Rafiquzzaman, Review on in vivo and in vitro methods evaluation of antioxidant activity, Saudi Pharmaceutical Journal 21 (2013) 143–152.

DOI: 10.1016/j.jsps.2012.05.002

Google Scholar

[2] H. Li, C. Li, B. Xia, Y. Zhou, L. Lin, D. Liao, A chemotaxonomic study of phytochemicals in Hedyotis corymbosa, Biochemical Systematics and Ecology 62 (2015) 173e177 62 (2015) 173-177.

DOI: 10.1016/j.bse.2015.06.028

Google Scholar

[3] M. Moniruzzaman, A. Ferdous, S. Irin, Evaluation of antinociceptive effect of ethanol extract of Hedyotis corymbosa Linn. whole plant in mice, Journal of Ethnopharmacology 161 (2015) 82–85.

DOI: 10.1016/j.jep.2014.12.011

Google Scholar

[4] R. Ahmad, A.M. Ali, D.A. Israf, N.H. Ismail, K. Shaari, N.H. Lajis, Antioxidant, radical-scavenging, anti-inflammatory, cytotoxic and antibacterial activities of methanolic extracts of some Hedyotis species, Life Sciences 76(17) (2005) 1953-1964.

DOI: 10.1016/j.lfs.2004.08.039

Google Scholar

[5] R.K. Gupta, R.K. Singh, S.R. Swain, T. Hussain, C.V. Rao3, Anti–hepatotoxic potential of Hedyotis corymbosa against D–galactosamine–induced hepatopathy in experimental rodents, Asian Pacific Journal of Tropical Biomedicine 2(3) (2012) S1542-S1547.

DOI: 10.1016/s2221-1691(12)60450-x

Google Scholar

[6] S. Sadasivan, P.G. Latha, J.M. Sasikumar, S. Rajashekaran, S. Shyamal, V.J. Shine, Hepatoprotective studies on Hedyotis corymbosa (L.) Lam, Journal of Ethnopharmacology 106(2) (2006) 245-249.

DOI: 10.1016/j.jep.2006.01.002

Google Scholar

[7] M.-C. Wei, S.-J. Hong, Y.-C. Yang, Isolation of triterpenic acid-rich extracts from Hedyotis corymbosa using ultrasound-assisted supercritical carbon dioxide extraction and determination of their fictitious solubilities, Journal of Industrial and Engineering Chemistry 48 (2017) 202-211.

DOI: 10.1016/j.jiec.2017.01.003

Google Scholar

[8] K. Mishra, A.P. Dash, B.K. Swain, N. Dey, Anti-malarial activities of Andrographis paniculata and Hedyotis corymbosa extracts and their combination with curcumin, Malaria Journal 8(26 ) (2009) 1-9.

DOI: 10.1186/1475-2875-8-26

Google Scholar

[9] L. López-Hortas, P. Pérez-Larrán, M.J. González-Muñoz, E. Falqué, H. Domínguez, Recent developments on the extraction and application of ursolic acid. A review, Food Research International 103 (2018) 130-149.

DOI: 10.1016/j.foodres.2017.10.028

Google Scholar

[10] R.A. Furtado, É.P. Rodrigues, F.R.R. Araújo, W.L. Oliveira, M.A. Furtado, M.B. Castro, W.R. Cunha, A.D.C. Tavares, Ursolic Acid and Oleanolic Acid Suppress Preneoplastic Lesions Induced by 1,2-Dimethylhydrazine in Rat Colon, Toxicologic Pathology 36 (2008) 576-580.

DOI: 10.1177/0192623308317423

Google Scholar

[11] Y.-C. Yang, M.-C. Wei, A combined procedure of ultrasound-assisted and supercritical carbon dioxide for extraction and quantitation oleanolic and ursolic acids from Hedyotis corymbosa, Industrial Crops and Products 79 (2016) 7-17.

DOI: 10.1016/j.indcrop.2015.10.038

Google Scholar

[12] N.H.J. Lajis, R. Ahmad, Phytochemical Studies and Pharmacological Activities of Plants in Genus Hedyotis/Oldenlandia, in: R. Atta ur (Ed.), Studies in Natural Products Chemistry, Elsevier2006, pp.1057-1090.

DOI: 10.1016/s1572-5995(06)80046-3

Google Scholar

[13] J.P. van der Eerden, Relevance of surface roughening and surface melting for crystal growth, Journal of Crystal Growth 128(1) (1993) 62-68.

DOI: 10.1016/0022-0248(93)90296-9

Google Scholar

[14] X.S. Noundou, R.W.M. Krause, S.F.v. Vuuren, D.T. Ndinteh, D.K. Olivier, Antibacterial effects of Alchornea cordifolia (Schumach. and Thonn.) Müll. Arg extracts and compounds on gastrointestinal, skin, respiratory and urinary tract pathogens, Journal of Ethnopharmacology 179 (2016) 76-82.

DOI: 10.1016/j.jep.2015.12.043

Google Scholar

[15] A. Pielesz, A. Gawłowski, D. Biniaś, R. Bobiński, M. Kawecki, A. Klama-Baryła, D. Kitala, W. Łabuś, J. Glik, J. Paluch, The role of dimethyl sulfoxide (DMSO) in ex-vivo examination of human skin burn injury treatment, Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy 196(5) (2018) 344-352.

DOI: 10.1016/j.saa.2018.02.035

Google Scholar

[16] A. Doğan, S. Otlu, Ö. Çelebİ, P.A. Kiliçle, A.G. Sağlam, A.N.C. Doğan, N. Mutlu, An investigation of antibacterial effects of steroids, Turkish Journal of Veterinary and Animal Sciences 41 (2017) 302-305.

DOI: 10.3906/vet-1510-24

Google Scholar