Determination of Silver Nanoparticle Concentration Ratio in the Blood and Brain of Rats for Different Administration Routes

Abstract:

A study to assess the concentrations ratio of silver nanoparticles (Ag-NPs) in the blood and brain of the male Wistar rats using nuclear physical methods has been carried out. The Ag-NPs suspension including quasi-spherical Ag-NPs was administrated intravenously (in the tail vein for Ag-NPs of 9+2 nm and 94+10 nm), and for Ag-NPs of 94 nm diameter it was also administrated orally and intratracheally. The organ recovery was made in 24 h following the administration (for three types of administration) and one more time in 120 h in case of intravenous administration. Radioactive label in the nuclei of silver was created by irradiation of Ag-NPs suspensions in a flow of reactor thermal neutrons, and its share was 5.6 ∙ 10^-7 of the total number of silver atoms. This fact could not affect the overall physical and chemical properties of radiolabeled Ag-NPs. We measured the activity of the ^110mAg isotope-label in samples of blood and brain of rats, while the activity of ⁵⁹Fe isotope was measured after the exposure to samples of these organs.Given the fact that iron is contained mainly in the hemoglobin of blood, on the basis of the measurement of ⁵⁹Fe activity being the induced by neutron flux, we derived an evaluation of the residual mass of blood in brain capillaries using a standard procedure to prepare the samples. This determination is 0.058±0.010 g, and on average this is about 0 37±0.09% of the total mass of blood in rats. The estimated ratio of the Ag-NPs concentration in brain samples of rats (minus the Ag-NPs number in residual blood capillaries) to their peripheral blood concentration for 9 nm Ag-NPs is 0.16±0.04 and 0.31±0.07, and for 94 nm Ag-NPs - 0.20±0.05 and 0.29±0.07 for times in 24 and 120 hours after intravenous administration, respectively. For Ag-NPs of 9 nm and 94 nm diameter we revealed no significant effect of the Ag-NPs size on the value of this ratio. The same ratio for Ag-NPs of 94 nm diameter in 24 h after the oral and intratracheal administration is 0.29+0.09 and 0.41+0.12, respectively.

• Periodical: Nano Hybrids and Composites (Volume 13)
• Pages: 206-210
• DOI: https://doi.org/10.4028/www.scientific.net/NHC.13.206
• Online since: January 2017
• Authors: Vladimir Yu. Soloviev*, Anna A. Antsiferova, Svetlana S. Fatkina, Vyacheslav A. Demin, Vladimir F. Demin
• Keywords: Biokinetics, Nuclear Physical Methods, Radioactive Tracer, Rats, Silver Nanoparticles

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