Acoustic underwater positioning and navigation systems still evolve in a few directions. Improvements in underwater navigation sensor technology and underwater navigation algorithms cause development of new underwater vehicles and new underwater vehicle missions. Up-to-date navigation multi-sensor systems based on USBL become very popular. This paper presents experimental investigation of hydroacoustic multi-sensor based on tetrahedral antenna configuration. The preliminary investigations were led in water tank as first phase before multi-sensor measurement in real conditions, in sea water environment.