Metagenome-Derived Draft Genome Sequence of Acidithiobacillus ferrooxidans RV1 from an Abandoned Gold Tailing in Neuquén, Argentina

Abstract:

In this work we report the metagenome-derived draft genomic sequence of an enrichment culture dominated by A. ferrooxidans obtained from an airlift bioreactor inoculated with the microbial consortium recovered from the “Relave Viejo” tailing. The genome of this culture was assembled de-novo and by reference, generating a consensus assembly of 3.0 Mb. On the basis of 16S rRNA (100 % identity), average nucleotide identity analysis (99.33% identity) and in silico DNA-DNA hybridization against A. ferrooxidans ATCC 23270^T (97.9%), the recovered genome is confirmed to pertain to A. ferrooxidans species. Comparative genomics results are presented to uncover the genetic traits of the variant surviving lime treatment and to further explore the genomic diversity of these model iron oxidizing species.

• Periodical: Solid State Phenomena (Volume 262)
• Pages: 439-442
• DOI: https://doi.org/10.4028/www.scientific.net/SSP.262.439
• Online since: August 2017
• Authors: Ricardo Ulloa, Ana Moya-Beltrán, Francisco Issotta, Harold Nuñez, Paulo C. Covarrubias, Edgardo R. Donati, Raquel Quatrini*, Alejandra Giaveno
• Keywords: Abandoned Tailing, Acidithiobacillus ferrooxidans RV1, Gold Mine, Lime Treatment, Northern Patagonia

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