Molecular Response of the Acidophilic Iron Oxidizer “Ferrovum” sp. JA12 to the Exposure to Elevated Concentrations of Ferrous Iron

Sophie R. Ullrich; Anja Poehlein; Gloria J. Levicán; Michael Schlömann; Martin Mühling

doi:10.4028/www.scientific.net/SSP.262.482

Paper Titles

Identification of Sulfur Activation Relevant Protein Genes of Extremely Thermophilic Acidianus manzaensis
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Evolution of Compositions and Contents of Capsule and Slime EPSs for Adaptation to and Action on Energy Substrates and Heavy Metals by Typical Bioleaching Microorganisms
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Resistance of Moderately Thermophilic Acidophilic Microorganisms to Ferric Iron Ions
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Effect of Galactose on EPS Production and Attachment of Acidithiobacillus thiooxidans to Mineral Surfaces
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Molecular Response of the Acidophilic Iron Oxidizer “Ferrovum” sp. JA12 to the Exposure to Elevated Concentrations of Ferrous Iron
p.482

The Surface Chemistry Characterization of Pyrite, Sphalerite and Molybdenite after Bioleaching
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Monitoring of Biofilm Development on Surfaces Using an Electrochemical Method
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EIS Studies of Chalcopyrite Involving Iron(II) Ions
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Thermochelin, a Hydroxamate Siderophore from Thermocrispum agreste DSM 44070
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Molecular Response of the Acidophilic Iron Oxidizer “Ferrovum” sp. JA12 to the Exposure to Elevated Concentrations of Ferrous Iron

Abstract:

The response to elevated ferrous iron concentrations was investigated in the acidophilic iron oxidizer “Ferrovum” sp. JA12 at transcriptome level. Detoxification of reactive oxygen species appears to be the most important strategy to cope with oxidative stress. The proposed iron oxidation model in “Ferrovum” spp. was supported by the transcriptome data of “Ferrovum” sp. JA12. Several gene candidates of the iron oxidation model are organized in a gene cluster conserved in iron oxidizing betaproteobacteria and zetaproteobacteria possibly indicating a common origin of iron oxidation.

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Solid State Phenomena (Volume 262)

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482-486

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https://doi.org/10.4028/www.scientific.net/SSP.262.482

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Online since:

August 2017

Authors:

Sophie R. Ullrich*, Anja Poehlein, Gloria J. Levicán, Michael Schlömann, Martin Mühling

Keywords:

Ferrovum, Microbial Iron Oxidation, Oxidative Stress, RNA-Seq, Transcriptome

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References

[1] W.J. Ingledew, Thiobacillus ferrooxidans. The bioenergetics of an acidophilic chemolithotroph, Biochim. Biophys. Acta 683 (1982) 89–117.

DOI: 10.1016/0304-4173(82)90007-6

Google Scholar

[2] L.J. Bird, V. Bonnefoy, D.K. Newman, Bioenergetic challenges of microbial iron metabolisms, Trends Microbiol. 19 (2011) 330–340.

DOI: 10.1016/j.tim.2011.05.001

Google Scholar

[3] J.A. Imlay, The molecular mechanisms and physiological consequences of oxidative stress: lessons from a model bacterium, Nat. Rev. Microbiol. 11 (2013) 443–454.

DOI: 10.1038/nrmicro3032

Google Scholar

[4] J.P. Cárdenas, F. Moya, P. Covarrubias, A. Shmaryahu, G. Levicán, D.S. Holmes, R. Quatrini, Comparative genomics of the oxidative stress response in bioleaching microorganisms, Hydrometallurgy 127-128 (2012) 162–167.

DOI: 10.1016/j.hydromet.2012.07.014

Google Scholar

[5] S.R. Ullrich, A. Poehlein, J.S. Tischler, C. González, F.J. Ossandon, R. Daniel, D.S. Holmes, M. Schlömann, M. Mühling, Genome analysis of the biotechnologically relevant acidophilic iron oxidising strain JA12 indicates phylogenetic and metabolic diversity within the novel genus Ferrovum, PLoS ONE 11(2016a) e0146832.

DOI: 10.1371/journal.pone.0146832

Google Scholar

[6] J.S. Tischler, R.J. Jwair, N. Gelhaar, A. Drechsel, A. Skirl, C. Wiacek, E. Janneck, M. Schlömann, New cultivation medium for Ferrovum, and Gallionella-related strains, J. Microbiol. Methods 95 (2013) 138–144.

DOI: 10.1016/j.mimet.2013.07.027

Google Scholar

[7] S.R. Ullrich, A. Poehlein, S. Voget, M. Hoppert, R. Daniel, J.S. Tischler, M. Schlömann, M. Mühling, Permanent draft genome sequence of Acidiphilium sp. JA12-A1, Stand. Genomic Sci. 10 (2015) 56.

DOI: 10.1186/s40793-015-0040-y

Google Scholar

[8] A.M. Bolger, M. Lohse, B. Usadel, Trimmomatic: a flexible trimmer for Illumina sequence data, Bioinformatics 30 (2014) 2114–2120.

DOI: 10.1093/bioinformatics/btu170

Google Scholar

[9] B. Langmead, S.L. Salzberg, Fast gapped-read alignment with Bowtie 2, Nat. Methods 9 (2012) 357–359.

DOI: 10.1038/nmeth.1923

Google Scholar

[10] S. Wiegand, S. Dietrich, R. Hertel, J. Bongaerts, S. Evers, S. Volland, R. Daniel, H. Liesegang, RNA-Seq of Bacillus licheniformis: active regulatory RNA features expressed within a productive fermentation, BMC Genomics 14 (2013) 667.

DOI: 10.1186/1471-2164-14-667

Google Scholar

[11] A. Mortazavi, B.A. Williams, K. McCue, L. Schaeffer, B. Wold Mapping and quantifying mammalian transcriptomes by RNA-Seq, Nat. Methods 5 (2008) 621–628.

DOI: 10.1038/nmeth.1226

Google Scholar

[12] S.R. Ullrich, C. González, A. Poehlein, J.S. Tischler, R. Daniel, D.S. Holmes, M. Schlömann, M. Mühling. Gene loss and horizontal gene transfer contributed to the genome evolution of the extreme acidophile Ferrovum, Front. Microbiol. 7 (2016b) e78237.

DOI: 10.3389/fmicb.2016.00797

Google Scholar

[13] D.B. Johnson, K.B. Hallberg, S. Hedrich, Uncovering a microbial enigma: isolation and characterization of the streamer-generating, iron-oxidizing, acidophilic bacterium Ferrovum myxofaciens, Appl. Environ. Microbiol. 80 (2014) 672–680.

DOI: 10.1128/aem.03230-13

Google Scholar

[14] A. Amouric, C. Brochier-Armanet, D.B. Johnson, V. Bonnefoy, K.B. Hallberg, Phylogenetic and genetic variation among Fe(II)-oxidizing acidithiobacilli supports the view that these comprise multiple species with different ferrous iron oxidation pathways, Microbiology 157 (2011).

DOI: 10.1099/mic.0.044537-0

Google Scholar