Identification of Sulfur Activation Relevant Protein Genes of Extremely Thermophilic Acidianus manzaensis

Abstract:

The sulfur activation by extracellular proteins is considered as the crucial stage during biooxidation of elemental sulfur (S⁰). In order to study genes encoding sulfur-activation related extracellular proteins of extremely thermophilic Acidianus manzaensis, the extracellular proteins with higher abundance for the strain grown on S⁰ allotropes than that on Fe²⁺ were first screened by two-dimensional gel electrophoresis, and then identified by matrix-assisted laser desorption ionization time-of-flight mass spectrometry. Nine genes amplified with PCR were satisfactory according to their agarose gel electrophoresis. The differential expression of these nine genes when the strain grown on S⁰ allotropes and Fe²⁺ were analyzed with real-time quantitative PCR (RT-qPCR). Results showed that seven of them were higher expressed when the strain grown on S⁰ allotropes than on Fe²⁺, indicating they may be related with sulfur activation by A. manzaensis.

• Periodical: Solid State Phenomena (Volume 262)
• Pages: 461-465
• DOI: https://doi.org/10.4028/www.scientific.net/SSP.262.461
• Online since: August 2017
• Authors: Hong Chang Liu, Jin Lan Xia*, Zhen Yuan Nie, Ya Long Ma, Yun Yang, Li Zhu Liu, Xuan Pan, Peng Yuan
• Keywords: Acidianus manzaensis, Comparative Proteomics, RT-qPCR, Sulfur Activation

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