Revisiting the Chrome Azurol S Assay for Various Metal Ions

Abstract:

Siderophores play an important role in the solubilisation and mobilization of iron (III) and various metal ions. To have a useful method to test siderophores in culture supernatants for their metal binding affinity, we redesigned and optimized the liquid CAS-assay for selected metal ions. CAS-assay solutions were calibrated with desferrioxamine B in different concentrations to calculate DFOB-equivalents to get a semi-quantitative evaluation. With these assay solutions, we were able to test siderophores in culture supernatants for their ability to chelate with Fe, Al, Ga, Cu, V and As.

• Periodical: Solid State Phenomena (Volume 262)
• Pages: 509-512
• DOI: https://doi.org/10.4028/www.scientific.net/SSP.262.509
• Online since: August 2017
• Authors: Marika Mehnert*, Gerardo Retamal-Morales, Rïngo Schwabe, Sabine Vater, Thomas Heine, Gloria J. Levicán, Michael Schlömann, Dirk Tischler
• Keywords: Chrome Azurol S, Metal Binding, Metal Chelating Agents, Siderophore, Soil Bacteria

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