Siderophore Purification via Immobilized Metal Affinity Chromatography

Abstract:

Siderophores are low-molecular weight compounds that are produced by organisms to assimilate vital Fe³⁺ out of iron-deficient environments. They are of interest for several (bio-) technological applications because of their high selectivity for several metal ions. Unfortunately, the concentration in supernatants is often low and thus it is challenging to purify or even enrich these compounds. We applied different types of siderophores onto an immobilized metal-resin that was loaded with either Ni²⁺, Co²⁺ or Fe³⁺. Elution was done with ethanol to reduce salt load and facilitate downstream processing. Thus, it is possible to enrich as well as desalt a sample within one-step from culture supernatant, which allows faster characterization and application of siderophores.

• Periodical: Solid State Phenomena (Volume 262)
• Pages: 505-508
• DOI: https://doi.org/10.4028/www.scientific.net/SSP.262.505
• Online since: August 2017
• Authors: Thomas Heine*, Marika Mehnert, Rïngo Schwabe, Dirk Tischler
• Keywords: Desferrioxamine B, Ferrichrome, IMAC, Pyoverdine, Siderophore Purification

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