On the Immobilization of Desferrioxamine-Like Siderophores for Selective Metal Binding

Abstract:

Gordonia rubripertincta CWB2 produces hydroxamate-type siderophores. Therefore it was cultivated under iron limitation. Analytical reversed-phase HPLC allowed determining a single peak of ferric iron chelating compounds from culture broth. The elution profile and its absorbance spectrum were similar to those of desferrioxamine B. The latter is a commercial available metal chelating agent which is of interest for industries. We successfully developed an HPLC protocol to separate metal-free and metal-loaded desferrioxamines. Further, we aimed to increase the re-usability of desferrioxamines as metal chelators by immobilization on silica based carriers. The siderophores of strain CWB2 have been covalently linked to the carrier with a high yield (up to 95%). Metal binding studies demonstrated that metals can be bound to non-immobilized as well as to the covalently linked desferrioxamines.

• Periodical: Solid State Phenomena (Volume 262)
• Pages: 517-520
• DOI: https://doi.org/10.4028/www.scientific.net/SSP.262.517
• Online since: August 2017
• Authors: Marlene Kirstin Anke, Katarzyna Szymańska, Rïngo Schwabe, Oliver Wiche, Dirk Tischler*
• Keywords: Actinobacteria, Chelating Agent, DFOB, Gordonia, Immobilization, Metallophore

References

[1] R.C. Hider, X. Kong, Chemistry and biology of siderophores, Nat. Prod. Rep. 27 (2010) 637-657.

DOI: 10.1039/b906679a

[2] A. Evers, R.D. Hancock, A.E. Martell, R.J. Motekaitis, Metal ion recognition in ligands with negatively charged oxygen donor groups. Complexation of Fe(III), Ga(III), In(III), Al(III), and other highly charged metal ions, Inorg. Chem. 28 (1989).

DOI: 10.1002/chin.198938082

[3] H. -J. Kraemer, H. Breithaupt, Quantification of desferrioxamine, ferrioxamine and aluminoxamine by post-column derivatization high-performance liquid chromatography, J. Chromatogr. B. 710 (1998) 191-204.

DOI: 10.1016/s0378-4347(98)00093-0

[4] C.O. Esuola, O.O. Babalola, T. Heine, R. Schwabe, M. Schlömann, D. Tischler, Identification and characterization of a FAD-dependent putrescine N-hydroxylase (GorA) from Gordonia rubripertincta CWB2, J. Mol. Catal. B-Enzym. 134 (2016) 378-389.

DOI: 10.1016/j.molcatb.2016.08.003

[5] D.B. Alexander, D.A. Zuberer, Use of chrome azurol S reagents to evaluate siderophore production by rhizosphere bacteria, Biol. Fert. Soils 12 (1991) 39-45.

DOI: 10.1007/bf00369386

[6] O. Wiche, D. Tischler, C. Fauser, J. Lodeman, H. Heilmeier, Effects of citric acid and the microbial siderophore desferrioxamine B (DFO-B) on the mobility of germanium (Ge) and rare earth elements (REEs) in soil and uptake in Phalaris arundinacea, Int. J. Phytoremediat. (accepted).

DOI: 10.1080/15226514.2017.1284752

[7] A. Drożdż, A. Chrobok, S. Baj, K. Szymańska, J. Mrowiec-Białoń, A.B. Jarzębski, The chemo-enzymatic Baeyer–Villiger oxidation of cyclic ketones with an efficient silica-supported lipase as a biocatalyst, App. Catal. A 467 (2013) 163– 170.

DOI: 10.1016/j.apcata.2013.07.009

[8] M. Oelschlägel, A. Riedel, A. Zniszczoł, K. Szymańska, A.B. Jarzebski, M. Schlömann, D. Tischler, Immobilization of an integral membrane protein for biotechnological phenylacetaldehyde production, J. Biotechnol. 174 (2014) 7-13.

DOI: 10.1016/j.jbiotec.2014.01.019

[9] J. Qi, M.K. Anke, K. Szymańska, D. Tischler, Immobilization of Rhodococcus opacus 1CP azoreductase to obtain azo dye degrading biocatalysts operative at acidic pH, Int. Biodeter. Biodegr. 118 (2017) 89-94.

DOI: 10.1016/j.ibiod.2017.01.027