Gallium Mobilization in Soil by Bacterial Metallophores

Abstract:

In the present study we explore the idea of biotechnologically produced metallophore mixtures as selective chelating compounds for economically valuable metals from various sources. A complex soil matrix with natural levels of metal mineralization was employed as a potential source of metals. We focused on gallium-chelating metallophore preparations of two soil bacteria (Gordonia rubripertincta CWB2 and Paracoccus denitrificans PD1222) which were compared to the commercially available desferrioxamine B (DFOB). As a reference, the binding of iron was analyzed. The herein described successful mobilization of metals such as gallium from soil provides first hints towards alternative strategies, such as phytomining, sensor development, or solvent extraction based on metallophores. The metallophore mixture produced by the strains showed best results at pH 8 and allowed to mobilize gallium about three times better as the pure commercially available DFOB.

• Periodical: Solid State Phenomena (Volume 262)
• Pages: 513-516
• DOI: https://doi.org/10.4028/www.scientific.net/SSP.262.513
• Online since: August 2017
• Authors: Rïngo Schwabe, Britta Obst, Marika Mehnert, Dirk Tischler, Oliver Wiche*
• Keywords: Actinobacteria, Chelating Agent, DFOB, Dissolution, Gordonia, Metallophore, Metals

References

[1] J. Schijf, E.A. Christenson, K.J. Potter, Different binding modes of Cu and Pb vs. Cd, Ni, and Zn with the trihydroxamate siderophore desferrioxamine B at seawater ionic strength, Mar. Chem. 173 (2015) 40-51.

DOI: 10.1016/j.marchem.2015.02.014

[2] K.M. Kuhn, J. L DuBois, P.A. Maurice, Strategies of aerobic microbial Fe acquisition from Fe-bearing montmorillonite clay, Geochim. Cosmochim. Ac. 117 (2013) 191-202.

DOI: 10.1016/j.gca.2013.04.028

[3] A.A. Simanova, P. Persson, J.S. Loring, Evidence for ligand hydrolysis and Fe(III) reduction in the dissolution of goethite by desferrioxamine-B, Geochim. Cosmochim. Ac. 74 (2010) 6706-6720.

DOI: 10.1016/j.gca.2010.08.037

[4] H. Boukhalfa, A.L. Crumbliss, Chemical aspects of siderophore mediated iron transport, BioMetals 15 (2002) 325-339.

[5] R.C. Hider, X. Kong, Chemistry and biology of siderophores, Nat. Prod. Rep. 27 (2010) 637-657.

DOI: 10.1039/b906679a

[6] O. Wiche, D. Tischler, C. Fauser, J. Lodeman, H. Heilmeier, Effects of citric acid and the microbial siderophore desferrioxamine B (DFO-B) on the mobility of germanium (Ge) and rare earth elements (REEs) in soil and uptake in Phalaris arundinacea, Int. J. Phytoremediat. (2017).

DOI: 10.1080/15226514.2017.1284752

[7] G.E. de Vries, N. Harms, J. Hoogendijk, A.H. Stouthamer, Isolation and characterization of Paracoccus denitrificans mutants with increased conjugation frequencies and pleiotropic loss of a (nGATCn) DNA-modifying property, Arch. Microbiol. 152 (1989).

DOI: 10.1007/bf00447011

[8] C.O. Esuola, O.O. Babalola, T. Heine, R. Schwabe, M. Schlömann, D. Tischler, Identification and characterization of a FAD-dependent putrescine N-hydroxylase (GorA) from Gordonia rubripertincta CWB2, J. Mol. Catal. B-Enzym. 134 (2016) 378-389.

DOI: 10.1016/j.molcatb.2016.08.003

[9] D.B. Alexander, D.A. Zuberer, Use of chrome azurol S reagents to evaluate siderophore production by rhizosphere bacteria, Biol. Fert. Soils. 12 (1991) 39-45.

DOI: 10.1007/bf00369386

[10] M. Mehnert, R. Schwabe, S. Vater, T. Heine, G. Retamal-Morales, G.J. Levicán, M. Schlömann and D. Tischler: submitted to Solid State Phenomena (2017).

DOI: 10.4028/www.scientific.net/ssp.262.509

[11] M.K. Anke, K. Szymańska, R. Schwabe, O. Wiche and D. Tischler: submitted to Solid State Phenomena (2017).