Integrated Sulfate Reduction and Biosorption Process for the Treatment of Mine Drainages

Abstract:

Sulfate is a pollutant present in the mining waste water and acid mine drainage. High levels of sulfate can generate important environmental problems. One of the alternatives proposed for the treatment of water with high levels of sulfate is the use of sulfate-reducing microorganisms. This work describes the synergistic combination of a treatment system for the removal of metals by biosorption with the strain Bacillus sp. NRRL-B-30881 to reduce the inhibiting concentration of metals in waters, followed by a new process of sulfate removal that uses a halotolerant sulfate-reducing microbial consortium. The results show that the sulfate reducing consortium can be cultured and is able to reduce the sulfate concentration using cheaper complex organic substrates like spirulina, cellulose and industrial starch. The sulfate reducing consortium was cultured on a bioreactor with Celite R-635, as support material. Using this bioreactor it was possible to reduce the sulfate concentration in the culture medium in batch or semi-continuous operation. An acid mine drainage was pretreated by lime and treated by biosortion in order to increase the pH and reduce the heavy metals concentration. Subsequently the remaining sulfate was removed by the developed process. This integrated biological process represents a more economical alternative for the removal of metal by biosortion and the removal of sulfate using a sulfate reducing consortium.

• Periodical: Solid State Phenomena (Volume 262)
• Pages: 582-586
• DOI: https://doi.org/10.4028/www.scientific.net/SSP.262.582
• Online since: August 2017
• Authors: Davor Cotoras*, Cristian Hurtado, Pabla Viedma
• Keywords: Acid Mine Drainage (AMD), Biosorption, Metals, Reduction, Sulfate, Sulfate Reducing Bacteria (SRB)

References

[1] S. Tait, W.P. Clark , J. Keller, D.J. Batstone, Removal of sulfate from high-strength wastewater by crystallisation, Water Res. 43 (2009) 762-772.

DOI: 10.1016/j.watres.2008.11.008

[2] J.A. Sousa, C.M. Plugge, A.J. Stams, M.F. Bijmans, Sulfate reduction in a hydrogen fed bioreactor operated at haloalkaline conditions, Water Res. 68 (2015) 67-76.

DOI: 10.1016/j.watres.2014.09.035

[3] V.P. Utgikar, H.H. Tabak, J.R. Haines, R. Govind, Quantification of toxic and inhibitory impact of copper and zinc on mixed cultures of sulfate-reducing bacteria, Biotechnol. Bioeng. 82 (2003) 306-312.

DOI: 10.1002/bit.10575

[4] A.H. Kaksonen, J.A. Puhakka, Sulfate reduction based bioprocesses for the treatment of acid mine drainage and the recovery of metals, Eng. Life Sci. 7 (2007) 541-564.

DOI: 10.1002/elsc.200720216

[5] T.W. Hao, P.Y. Xiang, H.R. Mackey, K. Chi, H. Lu, H.K. Chui, G.H. Chen, A review of biological sulfate conversions in wastewater treatment, Water Res. 65 (2014) 1-21.

DOI: 10.1016/j.watres.2014.06.043

[6] G. Boshoff, J. Duncan, P.D. Rose, The use of micro-algal biomass as a carbon source for biological sulphate reducing systems, Water Res. 38 (2004) 2659-2666.

DOI: 10.1016/j.watres.2004.03.031

[7] D. Cotoras, F. Valenzuela, M. Zarzar and P. Viedma, U.S. Patent 7, 479, 220. (2009).

[8] L.L. Barton, F.A. Tomei, Characteristics and activities of sulfate-reducing bacteria, in: L.L. Barton (Ed. ), Sulfate-reducing bacteria, Springer, New York, 1995, pp.1-32.

DOI: 10.1007/978-1-4899-1582-5_1

[9] D. Cotoras and P. Viedma, U.S. Patent 7, 951, 578. (2011).

[10] D. A. Eaton, L. S. Clesceri, 4500-S2-D. Methylene Blue Method in Standard Methods for the Examination of Water & Wastewater–American Public Health Association, (2005).

[11] D. A. Eaton, L. S. Clesceri, 4500-SO42-E. Turbidimetric Method in Standard Methods for the Examination of Water & Wastewater–American Public Health Association, (2005).

[12] M.A.M. Reis, J.S. Almeida, P.C. Lemos, M.J.T. Carrondo, Effect of hydrogen sulfide on growth of sulfate reducing bacteria, Biotechnol. Bioeng. 40 (1992) 593-600.

DOI: 10.1002/bit.260400506