Development of Metal Ion Binding Peptides Using Phage Surface Display Technology

Nora Schönberger; Sabine Matys; Katrin Flemming; Falk Lehmann; Franziska L. Lederer; Katrin Pollmann

doi:10.4028/www.scientific.net/SSP.262.591

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HomeSolid State PhenomenaSolid State Phenomena Vol. 262Development of Metal Ion Binding Peptides Using...

Development of Metal Ion Binding Peptides Using Phage Surface Display Technology

Abstract:

Phage surface display technology is a useful tool for the identification of biosorptive peptides. In this work it is used for the identification of cobalt, nickel and gallium binding peptides. We present methods for the enrichment of metal ion binding bacteriophage clones from a commercial phage display library. Metal ion selective peptides are suitable to separate as well as concentrate cobalt and nickel from copper black shale leaching products (EcoMetals project) and gallium from industrial waste waters (EcoGaIn project). In contrast to common capture methods of specific binding phage for solid materials the ionic species have to be immobilized prior to the bio-panning procedure. This was realized by chemical complexation of the metal ions using commercial complexing agents on porous matrices. Moreover, an option to harvest non elutable strong binding phage is proposed.

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Periodical:

Solid State Phenomena (Volume 262)

Pages:

591-595

DOI:

https://doi.org/10.4028/www.scientific.net/SSP.262.591

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Online since:

August 2017

Authors:

Nora Schönberger*, Sabine Matys, Katrin Flemming, Falk Lehmann, Franziska L. Lederer, Katrin Pollmann

Keywords:

Biopanning, Cobalt, Gallium, Immobilized Metal Ions, Metal Binding Peptides, Nickel, Phage Surface Display

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