Study on the Size Dependence of AuNPs in Enhancement Radiation Effect for Superficial Kilovoltage X-Rays

Abstract:

Radiation therapy and chemotherapy remain the most widely used treatment options in treating cancer. Recent developments in cancer research show that therapy combined with high-atomic number materials such as gold nanoparticles (AuNPs) is a new way to treat cancer, in which AuNPs are injected through intravenous administration and bound to tumor sites has enhanced tumor cell killing. Radiation therapy aims to deliver a high therapeutic dose of ionizing radiation to the tumor without exceeding normal tissue tolerance. In this work AuNPs have been used for the enhancement of radiation effects on breast cancer cells (MCF-7) for superficial kilovoltage X-ray radiation therapy. The use of AuNPs in superficial kilovoltage X-ray beams radiation therapy will provide a high probability for photon interaction by photoelectric effect. These provide advantages in terms of radiation dose enhancement. In this work, MCF-7 cells were seeded in the 96-well plate and treated with 13 nm, 50 nm and 70 nm AuNPs before they were irradiated with 80 kVp X-rays beam at various radiation doses. Photoelectric effect is the dominant process of interaction of 80 kVp X-rays with AuNPs. When the AuNPs are internalized into the MCF-7 cells, the dose enhancement effect is observed. The presence of AuNPs in the MCF-7 cells will produce a higher number of photoelectrons, and resulting more “free radicals” that will lead to increase in cell death. Then, these free radicals will lead to DNA damage to the MCF-7 cells. To validate the enhanced killing effect, both with and without AuNPs MCF-7 cells is irradiated simultaneously. By comparison, the results show that AuNPs significantly enhance cancer killing and the enhancement radiation effect was dependent on the size of AuNPs.

• Periodical: Solid State Phenomena (Volume 290)
• Pages: 81-86
• DOI: https://doi.org/10.4028/www.scientific.net/SSP.290.81
• Online since: April 2019
• Authors: Nur Shafawati binti Rosli*, Azhar Abdul Rahman, Azlan Abdul Aziz, Shaharum Shamsuddin, Suhana Arshad
• Keywords: AuNPs, Kilovoltage X-Ray, MCF-7 Cells

References

[1] International Agency for Research on Cancer. (2014). World Cancer Report (2014).

[2] J. Bernier, E. J. Hall, and A. Giaccia, Radiation Oncology : A Century of Achievements, Nat. Rev. Cancer, vol. 4, (2004).

[3] T. Kong, J. Zeng, X. Wang, X. Yang, J. Yang, S. McQuarrie, A. McEwan, W. Roa, J. Chen, and J. Z. Xing, Enhancement of Radiation Cytotoxicity in Breast-Cancer Cells by Localized Attachment of Gold Nanoparticles, Small, vol. 4, no. 9, p.1537–43, Sep. (2008).

DOI: 10.1002/smll.200700794

[4] F. M. Khan, The Physics Of Radiation Therapy. (2003).

[5] D. Y. Joh, L. Sun, M. Stangl, A. Al Zaki, S. Murty, P. P. Santoiemma, J. J. Davis, B. C. Baumann, M. Alonso-basanta, D. Bhang, G. D. Kao, A. Tsourkas, and J. F. Dorsey, Selective Targeting of Brain Tumors with Gold Nanoparticle-Induced Radiosensitization, PLoS One, vol. 8, no. 4, (2013).

DOI: 10.1371/journal.pone.0062425

[6] J. F. Hainfeld, H. M. Smilowitz, M. J. O. Connor, F. Avraham, and D. N. Slatkin, Gold Nanoparticle Imaging and Radiotherapy of Brain Tumors in Mice, Nanomedicine, vol. 8, no. 10, p.1601–1609, (2013).

DOI: 10.2217/nnm.12.165

[7] S. Jain, J. A. Coulter, A. R. Hounsell, T. Karl, S. J. McMahon, W. B. Hylan, M. F. Muir, G. R. Dickson, K. M. Prise, F. J. Currell, J. M. O'Sullivan, and D. G. Hirst, Cell-Specific Radiosensitization by Gold Nanoparticles at Megavoltage Radiation Energies, Int J Radiat Oncol Biol Phys, vol. 79, no. 2, p.531–539, (2011).

DOI: 10.1016/j.ijrobp.2010.08.044

[8] Y. Lin, S. J. Mcmahon, M. Scarpelli, H. Paganetti, and J. Schuemann, Comparing Gold Nano-Particle Enhanced Radiotherapy with Protons, Megavoltage Photons and Kilovoltage Photons : A Monte Carlo, Phys. Med. Biol., vol. 59, p.7675–7689, (2014).

DOI: 10.1088/0031-9155/59/24/7675

[9] S. R. Makhsin, K. A. Razak, A. A. Aziz, and R. Noordin, Study on Controlled Size, Shape and Dispersity of Gold Nanoparticles (AuNPs) Synthesized via Seeded-Growth Technique for Immunoassay Labeling, Adv. Mater. Res., vol. 364, p.504–509, Oct. (2011).

DOI: 10.4028/www.scientific.net/amr.364.504

[10] N. S. Rosli, A. Abdul Rahman, A. A. Azlan, and S. Shamsuddin, Determining the Size and Concentration Dependence of Gold Nanoparticles in Vitro Cytotoxicity (IC50) Test using WST-1 Assay, AIP Proc., p.1–5, (2014).

DOI: 10.1063/1.4915189

[11] S. R. Makhsin, K. A. Razak, and R. Noordin, The Effects of Size and Synthesis Methods of Gold Nanoparticle-Conjugated MαHIgG4 for Use in An Immunochromatographic Strip Test to Detect Brugian Filariasis, Nanotechnology, vol. 23, (2012).

DOI: 10.1088/0957-4484/23/49/495719

[12] W. Haiss, N. T. K. Thanh, J. Aveyard, and D. G. Fernig, Determination of Size and Concentration of Gold Nanoparticles from UV-Vis Spectra, Anal. Chem., vol. 79, no. 11, p.4215–4221, (2007).

DOI: 10.1021/ac0702084

[13] W. N. Rahman, N. Bishara, T. Ackerly, C. F. He, P. Jackson, C. Wong, R. Davidson, and M. Geso, Enhancement of Radiation Effects by Gold Nanoparticles for Superficial Radiation Therapy, Nanomedicine Nanotechnology, Biol. Med., vol. 5, no. 2, p.136–142, Jun. (2009).

DOI: 10.1016/j.nano.2009.01.014