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Construction and Vaccine Studies on a Pseudorabies Virus with Deletions in Glycoprotein I and Glycoprotein E Genes* Songlin Zhang1,a,Meilin Jin2,3,b, Huanchun Chen2,3,b 1Shandong Binzhou Animal Science ＆Veterinary Medicine Academy, Binzhou 256600, China 2Unit of Animal Infectious Diseases, National Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, 430070, PR China 3Laboratory of Animal Virology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan430070, PR China ajlgzlzsl@qq.com,bjml8328@126.com Keywords: Deletion mutant; Homologous recombination; Pseudorabies virus (PRV); vaccine Abstract.
Materials and Methods A.Plasmid, cells and virus The parental virus, PRV Ea strain, a spreading strain, was originally isolated by our laboratory [8].
“Dissemination of wild-type and gC-, gE- and gI-deleted mutants of Aujeszky’s disease virus in the maxillary nerve and trigeminal ganglion of pigs after intranasal inoculation,” Journal of General Virology, vol. 76, Aug. 1995, pp. 2063-2066, doi: 10.1099/0022-1317-76-8-2063

Sun, “Comparison of DC Pollution Flashover Performance of Various Types of Long String Insulators,” Journal of Chongqing University(Natural Science Edition), vol. 30, no. 7, pp. 42-45, 2007(in Chinese)
Conf. on Properties and Application of Dielectric Materials, pp. 260-263, Beijing, China,1988 [17] R.

As for energy price, it is difficult to the statistical data on energy price, we select raw material, fuel and power purchasing price index of Beijing to replace energy price.
Based on the previous research result, we use the efficiency measure from the stage 1 DEA estimations as the dependent variables, and determinants of energy efficiency as explanatory variables, we can estimate the following Tobit regression model: (7) Where, is the annual energy efficiency, is the ratio of industrial added value to GDP, is the proportion of coal consumption in total energy consumption, is the raw material, fuel and power purchasing price index of Beijing, and is Beijing’s R&D expenditure.
Acknowledgements This work was financially supported by the Beijing Social Science Foundation (11JGB071).
Zhou, B.W.Ang and K.L.Poh: European Journal of Operational Research Vol. 189 (2008), p.1-18

Acknowledgments The work is performed within the state assignment of Russian Ministry of Education and Science (Assignment #2014/223, Project code #1347).
Lapin, Optimization of the process of nanoparticle fabrication by laser ablation of bulk targets in a liquid, Russian Physics Journal. 57, #3 (2015) In Print [12] R.
Lan, Nickel and cobalt nanoparticles produced by laser ablation of solids in organic solution, Materials Letters. 62 (2008) 1521-1524.

Experiment Materials.
Journal of Applied Polymer Science, Vol.102, 5848–5853, 2006 [7] Zhengsheng Wang.

In this paper, we take the 6408 series as an example, the material of inner race, outer race and balls is GCr15SiMn of which density is 7800kg/m3, Young's modulus is 2.08×105MPa and Poisson ratio is 0.29; the material of retainer is brass whose density is 8545kg/m3, Young's modulus is 1.06×10 5MPa and Poisson ratio is 0.324.
Journal of Automobile Engineering Science Vol. 216 (2002), p.455 [4] Di Sciuva M, Lomario D.A: Composite Structures 59 (2003), p. 155 [5] Manolis Papadrakakis, Nikos D Lagaros: Computer Methods in Applied Mechanics and Engineering 191(2002), p. 34 [6] Mainak Mazumdar, David W Coit, Kelvin MeBride: Quality Control and Applied Statistics 6 (2001), p.109

Because many for history carry records, material is unknown.
In 1989, Tongjing of Yubei Chongqing 5.4 on the Richter scale have detailed investigation material, more research in the earthquake.
Acknowledgements This work was financially supported by the Chongqing Municipal Science and Technology Project, Project No.
Earthquake journal, 2003, 25 (2): 199 ~ 204.

Homing and Docking Autopilot Design for Autonomous Underwater Vehicle // Applied Mechanics and Materials Vols. 490-491 (2014).
[16] Pshikhopov, V.Kh., Krukhmalev, V.A., Medvedev, M.Yu., Budko, A.Yu., Chufistov, V.M., Adaptive control system design for robotic aircrafts, 2013 IEEE Latin American Robotics Symposium, LARS 2013, doi:10.1109/LARS.2013.59 [17] Rutkovsky V.Yu., Sukhanov V.M., Glumov V.M., “Algorithm of Adaptation in the Attitude Control System of a Flexible Spacecraft”, 9th International Conference on Mathematical Problems in Engineering, Aerospace and Sciences (Icnpaa 2012), AIP Conference Proceedings, 1493, ed. 
Sukhanov Appliance of the concept of adaptive control with reference model for task of monitoring of transmission shafts, Journal of Automatics and Telemechanics, 2003, № 5, P. 131–146 [20] Pshikhopov, V.Kh., Medvedev, M.Yu., Block design of robust control systems by direct Lyapunov method, 2011, IFAC Proceedings Volumes (IFAC-PapersOnline), doi: 10.3182/20110828-6-IT-1002.00006

The rising family business is becoming an important parts of socialist market economy, it provides amount of commodities and labor sevice for the society, meets people’s growing needs on material and culture; promotes structural adjustment and economic growth at the same time with creating new demand; offers a large number of jobs, which reduces the employment pressure and ensures the social stability.
As shown in the "Survey of Chinese private enterprises", Academy of Social Sciences, since the opening up, the family enterprises can be survived until now are only 20-30 percent of the whole, the average expectancy for the family enterprises is only 3 to 5 years.
Currently, the knowledge economy age characterized by high-technology of information technology, network technique, new material technology and etc., will fundamentally change the human’s economic activities in various fields.
Journal of Chongqing Technology and Business University (West Forum), Jun, 2005, 15(3)

R01-2000-00375 from the Korea Science & Engineering Foundation.
Shah: Journal of Acoustic Emission Vol. 10 (1992), p. 97
Ohtsu: Construction and Building Materials Vol. 15 (2001), p. 311