In this paper, flow characteristics of water-based Al2O3 nanofluids according to nanoparticles shape are experimentally investigated in fully developed laminar flow regime. Al2O3 nanofluids of 0.3 Vol. % with sphere-, rod-, blade-, platelet-and brick-shaped nanoparticles are manufactured by the two-step method. Nanoparticles shape dispersed in base fluid are also checked using TEM image. Zeta potential and sedimentation are measured to examine suspension and dispersion characteristics of Al2O3 nanofluids with nanoparticles of various shapes. Based on the experimental results, it is found that the pressure drop of Al2O3 nanofluids strongly depends on the shape of nanoparticles at the fixed volume fraction of 0.3%. We experimentally show that the pressure drop characteristics of Al2O3 nanofluids can be explained by both the surface area per unit mass and the size of nanoparticles which are related with the shape of nanoparticles.