DNA Vaccines Co-Expressing Containing Hsp70 and GP5/M Protein of Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) Display Enhanced Immunogenicity

Abstract:

Construction of Eukaryotic recombinant expression Vector pCI-ORF5-ORF6/ pCI-ORF5-ORF6B-Hsp70 Containing Hsp70 and PRRSV GP5/M (encoded by ORF5 and ORF6 genes), and to study its immune effect. After ifentified by enzyme analysis and nucleotide sequencing test,the repression vector plasmid was transfected into COS-7 cells. The transient expression protein was detected by indirect immunofluorescence assay (IFA) and Western-blotting. The immunogenicities of this DNA vaccine constructs were firstly investigated in a mouse moder. IFN-γ, IL-4 of cytokine, and the spleen T-lymphocyte subgroup quantity (CD4+/CD8+) were detected. The result shows that the recombinant plasmid pCI-ORF5-ORF6-Hsp70 could induce higher response of cellular immune responses and specific hummune responses in mouse, providing the clinical basic data and theoretical basis for success of the DNA vaccine development.

• Periodical: Applied Mechanics and Materials (Volumes 195-196)
• Pages: 452-458
• DOI: https://doi.org/10.4028/www.scientific.net/AMM.195-196.452
• Online since: August 2012
• Authors: Lan Zuo, Qi Gui Yan, Yan Lei, Xu Wang, Ying Chun Feng, Yu Chen, Yu Peng Ren, Li Wan, Shuang Jiang Li, Bing Liu
• Keywords: DNA Vaccine, Hsp70, ORF5, ORF6, PRRSV

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