Using Immortalized Human Lymphocytes Cell Lines to Screen the Genetic Markers of ADH5 to Formaldehyde

Abstract:

Formaldehyde is a carcinogen with highly toxic effect on organisms. ADH5 gene encodes the human formaldehyde dehydrogenase and is important to the detoxification of formaldehyde. The purpose of this work was to establish a platform to study ADH5 genetic markers induced by formaldehyde. After analyzing ADH5 SNPs database of Chinese Population, the three specific SNP sites : rs1154409,rs28730619,rs1154414 were sequenced in Human immortalized B lymphocytes of Chinese Han population by DNA sequencing. The real-time reverse transcription polymerase chain reaction (RT-PCR) approach was used to estimate expression of ADH5 mRNAs influenced by SNPs structure in human immortalized lymphocytes. Different immortalized B lymphocytes lines are gained by genotyping of multiple SNP sites of ADH5 gene. The mRNA expression of ADH5 could up regulate by 0.0005% formaldehyde for 48h exposure. But it showed rs1154409,rs28730619,rs1154414 SNPs has no association with the expression of ADH5 mRNA induced by formaldehyde. The human immortality lymphocytes model might be an efficient tool in a detection of genetic biomarkers of susceptibility to chemicals. The further research should be explored in the protein expression in more cell lines.

• Periodical: Applied Mechanics and Materials (Volumes 195-196)
• Pages: 465-468
• DOI: https://doi.org/10.4028/www.scientific.net/AMM.195-196.465
• Online since: August 2012
• Authors: Juan Zhang, Li Hong Yin, Yue Pu Pu
• Keywords: ADH5, Formaldehyde, Human Immortalized Lymphocytes, mRNA Expression, SNP

References

[1] VJ Cogliano, Y Grosse, RA Baan, MB Secretan, F El Ghissassi. Meeting report: summary of IARC monographs on formaldehyde, 2-butoxyethanol, and 1-tert-butoxy-2-propanol, Environ Health Perspect., Vol. 113, pp.1205-1208, (2005).

DOI: 10.1289/ehp.7542

[2] M Weng, L Zhu, K Yang, S Chen. Levels, sources, and health risks of carbonyls in residential indoor air in Hangzhou, China, Environ Monit Assess. Vol. 163, pp: 573-581, (2009).

DOI: 10.1007/s10661-009-0859-z

[3] Y Lu, J Liu, B Lu, A Jiang, C Wan. Study on the removal of indoor VOCs using biotechnology, J Hazard Mater. Vol. 182, pp.204-209, (2010).

DOI: 10.1016/j.jhazmat.2010.06.016

[4] HD Heck, M Casanova, TB Starr. Formaldehyde toxicity-new understanding, Toxicol, Vol. 20, pp.397-426, (1990).

[5] HS Kwon, MS Kim, HJ Edenberg, MW Hur. Sp3 and Sp4 can repress transcription by competing with Sp1 for the core cis-elements on the human ADH5/FDH minimal promoter, J Biol Chem., Vol. 274, pp.20-28, (1999).

DOI: 10.1074/jbc.274.1.20

[6] A Llopart, JM Comeron, FG Brunet, D Lachaise, M Long. Intron presence-absence polymorphism in Drosophila driven by positive Darwinian selection, Pnas, Vol. 99, pp.8121-8126, (2002).

DOI: 10.1073/pnas.122570299

[7] JW Watters, A Kraja, MA Meucci, MA Province, HL McLeod. Genome-wide discovery of loci influencing chemotherapy cytotoxicity, Proc Natl Acad Sci USA., Vol. 101, pp.11809-11814, (2004).

DOI: 10.1073/pnas.0404580101