Modification of PK-15 Cells Proteome in the early Stage of Classical Swine Fever Virus Infection

Abstract:

To uncover host cellular responses in the early stage of classical swine fever virus infection, a proteomic analysis was conducted using 2D electrophoresis followed by MALDI-TOF-TOF identification. Altered expression of 21 protein spots in infected pk-15 cells at 24 h p.i. were identified in 2D gels, with 13 of these being characterized by MALDI-TOF-TOF. These proteins function in cytoskeletal, energy metabolism, nucleic acid/processing, and cellular stress. The expression alteration of these proteins presents the changes in physiological functions of host cells and provides a clue for further understanding of the mechanisms of CSFV infection and pathogenesis.

• Periodical: Applied Mechanics and Materials (Volumes 195-196)
• Pages: 459-464
• DOI: https://doi.org/10.4028/www.scientific.net/AMM.195-196.459
• Online since: August 2012
• Authors: Jin Fu Sun
• Keywords: 2-DE, Classical Swine Fever Virus, Proteome

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