Calcium Ion and Protocorm Differentiation for Cymbidium Hybrid Cymbidium Lucky Gloria × Cymbidium Lovely Moon 'Crescent'

Chuan Gui Yang; Bao Gao; Jing Ran Liu

doi:10.4028/www.scientific.net/AMM.195-196.475

Paper Titles

DNA Vaccines Co-Expressing Containing Hsp70 and GP5/M Protein of Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) Display Enhanced Immunogenicity
p.452

Modification of PK-15 Cells Proteome in the early Stage of Classical Swine Fever Virus Infection
p.459

Using Immortalized Human Lymphocytes Cell Lines to Screen the Genetic Markers of ADH5 to Formaldehyde
p.465

Optimal Conditions for Cholesterol Oxidase Production and Enzymatic Properties Study Produced by Monascus X-1
p.469

Calcium Ion and Protocorm Differentiation for Cymbidium Hybrid Cymbidium Lucky Gloria × Cymbidium Lovely Moon 'Crescent'
p.475

Comparison of Multi Freedom Degrees for Patient Positioning Using Kilovoltage Cone Beam CT in Image-Guided Radiotherapy
p.480

The Design and Realization of the Networked Virtual Experiment Platform for Sensor Technology
p.487

Application of Fuzzy Enhancement in the Diagnosis of Liver Cancer from Ultrasound Images
p.493

A Semantic-Aware Context-Based Access Control Framework for Mobile Web Services
p.498

HomeApplied Mechanics and MaterialsApplied Mechanics and Materials Vols. 195-196Calcium Ion and Protocorm Differentiation for...

Calcium Ion and Protocorm Differentiation for Cymbidium Hybrid Cymbidium Lucky Gloria × Cymbidium Lovely Moon 'Crescent'

Abstract:

The low protocorm rate of the hybrid Cymbidium Lucky Gloria × Cymbidium Lovely Moon Crescent (LLC) has long been a detriment to its propagation. In this paper, we describe the thorough research about the effects of calcium ion concentration on the protocorm differentiation for Cymbidium hybrid LLC in vitro through 4 levels of Ca²⁺ concentration in MS medium based on the trial test including medium experiment with different calcium concentrations and the use of calcium inhibitor La³⁺. Our studies revealed that Ca²⁺ played a key role in protocorm differentiation of LLC, which was against the generally accepted idea about the function of 6-benzyl aminopurine (6-BA) and indole-3-butyric acid (IBA) in hybrid cymbidium protorcorm differentiation in vitro. The Chain reaction constituted by cytokinin, calcium, and auxin is submitted to exloringly explain the function mechanism of Ca²⁺.

You might also be interested in these eBooks

Mechanical Engineering and Intelligent Systems

View Preview

Info:

Periodical:

Applied Mechanics and Materials (Volumes 195-196)

Pages:

475-479

DOI:

https://doi.org/10.4028/www.scientific.net/AMM.195-196.475

Citation:

Cite this paper

Online since:

August 2012

Authors:

Chuan Gui Yang, Bao Gao, Jing Ran Liu

Keywords:

Calcium Ion, Cymbidium, Differentiation, In Vitro, Subculture

Export:

RIS, BibTeX

Price:

Permissions CCC:

Request Permissions

Permissions PLS:

Request Permissions

Сopyright:

Citation:

References

[1] C.F. Blidar, G. E. Bandici, I. Szabó, F. Codoban-Schiop, N. Groza-Ganea The reactivity of cymbidium hybridum protocorm-like bodys vitrocultivated in liquid medium, depleted in microelements, Analele Universâtii din Oradea, Fascicula Biologie Tom. XVI / 2, pp.25-31, (2009).

Google Scholar

[2] S. Gilroy, D. P. Blowers, A. J. Trewavas, Calcium: a regulation system emerges in plant cells, Development, no. 100, pp.181-184, (1987).

DOI: 10.1242/dev.100.2.181

Google Scholar

[3] Z. H. Fu, Rapid multiplication of orchid Cymbidium grandiflorium, Guangdong Agr. Sci. no. 3, pp.125-127, (2010).

Google Scholar

[4] P. K. Hepler, Calcium: a central regulator of plant growth and development, The Plant Cell, no. 17, pp.2142-2155, (2005).

DOI: 10.1105/tpc.105.032508

Google Scholar

[5] A. Hicks, Iron and calcium as micronutrients, The Orchid Review March/April: 89-91, (2008).

Google Scholar

[6] S.Y. Ju, X.E. Gao, Q.G. Chen, L. Li, G.B. Gao, Study on rapid multiplication techniques for Cymbidium hybridism J. Wuhan Ins. Tech. vol. 7, no. 5, pp.59-61, (2008).

Google Scholar

[7] X. L. Li, J. Yang, Y. J. Wu, Preliminary study on tissue culture of Cymbidium grandiflorum, J. Hubei Agr. Col. Vol. 24, no. 2, pp.272-274, (2004).

Google Scholar

[8] S. S. Medvedev, Calcium signaling system in plants, Russian Journal of Plant Physiology, vol. 52, no. 2, pp.249-270, (2005).

Google Scholar

[9] H. Miedema, J. H. F. Bothwell, C. Brownlee, J. M. Davies, Calcium uptake by plant cells-channels and pumps acting in concert, Tren. Plant Sci, vol. 16, no. 11, pp.514-519, (2001).

DOI: 10.1016/s1360-1385(01)02124-0

Google Scholar

[10] M. J. Saunders, P. K. Hepler, Calcium ionophore A23187 stimulates cytokinin-like mitosis in Funaria, Science, no. 217, pp.943-945, (1982).

DOI: 10.1126/science.217.4563.943

Google Scholar

[11] P. J. White, M. R. Broadley, Calcium in plants, Ann. Bot. no. 92, pp.487-511, (2003).

Google Scholar