Optimal Conditions for Cholesterol Oxidase Production and Enzymatic Properties Study Produced by Monascus X-1

Abstract:

In this paper we optimized liquid fermentation conditions of Monascus X-1 on cholesterol oxidase (COD) production, and then the COD was isolated by ammonium sulfate deposition and its properties were studied. The results showed that COD produced by Monascus X-1 was a kind of extracellular enzyme. The yields came to maximum at the 6^th day. The most suitable medium for Monascus X-1 generating COD was the rice medium, the highest enzyme activity was obtained at 140r/min, inoculum concentration 6%, when 60mL of the culture in 250mL shake flasks grown in rice medium. Under the optimized cultivation conditions, the enzyme activity reached 1168U/L, which increased 1.52 times compared to the previous fermentation conditions. The enzyme had favorable pH and thermal stability, while the optimum pH and temperature of enzyme activity was 7.0 and 40, respectively.

• Periodical: Applied Mechanics and Materials (Volumes 195-196)
• Pages: 469-474
• DOI: https://doi.org/10.4028/www.scientific.net/AMM.195-196.469
• Online since: August 2012
• Authors: Lu Hao, Qian Zhang, Chang Lu Wang, Mian Hua Chen, Yu Rong Wang, Feng Juan Li, Hua Yang, Jing Wang
• Keywords: Cholesterol Oxidase, Enzyme Property, Fermentation Conditions, Monascus X-1

References

[1] Turffitt G, The microbiological degradation of steroids, J. Bact. pp.447-87, (1994).

[2] Kociubinski G, Perez P, Antoni G, Screening of bile resistance and bile precipitation in lactic acid bacteria and Bifid bacteria, J food Prot, vol. 62, pp.905-912, (1999).

DOI: 10.4315/0362-028x-62.8.905

[3] De roos N M, Schouten G, Katane M B, Yogurt, Enriched with Lactobacillus acidophilus doesn't lower blood lipids in healthy men and women with normal to borderline high serum cholesterol levels, Eur J Clin Nutr, vol. 53, p.277, (1998).

DOI: 10.1038/sj.ejcn.1600722

[4] Kannel WB, Castelli WP, Gordon T, Cholesterol in the prediction of atherosclerotic disease: new perspectives based on the Framingham study And Intern med, vol. 90, no. 1, pp.85-91, (1979).

DOI: 10.7326/0003-4819-90-1-85

[5] Pekkanne J , Linn S , Heiss G, et al, Ten year mortality from cardiovascular disease in relation to cholesterol level among men with and without preexisting cardiovascular disease, New England J Med, vol. 322, pp.1700-1707, (1990).

DOI: 10.1056/nejm199006143222403

[6] Best D, New technology for cholesterol reduction, J of Food Processing , vol. 50 (12), pp.156-160, (1989).

[7] Deping Ye, Jiahong Lei, Wei Li, et al, Purification and characterization of extracellular cholesterol oxidase from Enterobacter sp, World J Microbiol Biotechnol, vol. 24, pp.2227-2233, (2008).

DOI: 10.1007/s11274-008-9734-2

[8] G.E. Turfitt, The microbiological degradation of steroids, Biochem J, vol. 38(5), pp.492-496, (1944).

[9] Myeong Soo Park, Bin Kwon, Jae Jung Shim, et al, Heterologous expression of cholesterol oxidase in Bifidobacterium longum under the control of 16S rRNA gene promoter of bifidobacteria, Biotechnol Lett, vol. 30, pp.165-172, (2008).

DOI: 10.1007/s10529-007-9514-3

[10] Laura Motteran, Mirella S, Pilone, Gianluca Molla, et al, Cholesterol Oxidase from Brevibacterium sterolicum, The Journal of Biological Chemistry, vol. 276, no. 21, pp.18024-18030, (2001).

DOI: 10.1074/jbc.m010953200

[11] M. Tabatabaei Yazdi, F. Malekzadeh, Gh. Zarrini, et al, Production of cholesterol oxidase by a newly isolated Rhodococcus sp, World Journal of Microbiology&Biotechnology, vol. 17, pp.731-737, (2001).

DOI: 10.1023/a:1012993532686

[12] Wenming Ji, Using colorimetric method to determine COD activity, Academic journal of Wuxi University of Light Industry, vol. 19, no. 3, pp.251-254, 2000 (in Chinese).