In this paper, the effect of laser radiation on the germination percentage and growth of soybean seed is introduced. The experimental result demonstrate that soybean seed various measurement value is optimum when the soybean seed was irradiated on the seed hilum for 10 minute, using He-Ne laser of 5mw. And the experimental results is also most significant when the soybean seed was irradiated on the seed hilum for 90 second, using He-Ne laser of 40mw. It is founded the experimental data is not significant if the dose of He-Ne laser is less and soybean growth is restrained if the dose of He-Ne laser is more. This shows proper dose of He-Ne laser of soybean seed conducted radiation, promoted soybean seed enzyme activity enhanced, its germination process, the nutrient supply full, thereby improving the soybean seed germination rate, soybean seedlings plant height and chlorophyll content. The results offer us a reference for laser radioactive breed and Laser Biology.