Silk Protein Assisted Ex Vivo Remineralization of Enamel-Like Microstructure

Abstract:

Based on the basic theory of molecular recognition, we designed an organic molecules model that spontaneously form three-dimensional fibrillar scaffolds to induce the crystallization of hydroxyapatite to synthesized enamel-like calcium phosphate/hydroxyapatite under a controllable way in vitro. Cross-linking of collagen on the dentin surface and silk fibroin with N,N-(3-dimethylaminopropyl)-N'-ethyl-carbodiimide hydrochloride (EDC) and N-hydroxysuccinimide (NHS) was optimized by varying the NHS/EDC molar ratio at constant EDC concentration. CaCl₂ and Na₃PO₄-12H₂O solution was added with Ca: P odd as 1.67:1 after conjugated. The results showed that the dentinal tubule were blocked by neonatal hydroxyapatite layer which has a continuous structure of columns crystal with size of 10-40nm. Furthermore, there were column crystal with parallel direction inside, similar to the crystal array in the top of enamel rod. The results suggest that silk protein monolayer may be useful in the modulation of mineral behavior during in situ dental tissue engineering.

• Periodical: Applied Mechanics and Materials (Volumes 66-68)
• Pages: 1682-1687
• DOI: https://doi.org/10.4028/www.scientific.net/AMM.66-68.1682
• Online since: July 2011
• Authors: Kun Tian, Min Peng, Ping Wu, Chu Hang Liao, Fa Yin Huang
• Keywords: Enamel, Hydroxyapatite (HAP), Silk Protein

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