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HomeApplied Mechanics and MaterialsApplied Mechanics and Materials Vol. 664The Investigation of the Interaction between...

The Investigation of the Interaction between Daidzin and Bovine Serum Albumin by Fluorescence Spectroscopy

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Abstract:

We investigated the mutual interaction of daidzin with bovine serum albumin (BSA) by fluorescence spectroscopy. The results revealed that daidzin cause the fluorescence quenching of BSA through a static quenching procedure. The Stern-Volmer quenching constant (K_sv) were calculated at different temperature. The binding site (n), apparent binding constant (K_a) and corresponding thermodynamic parameters △G^o, △H^o, △S^o were calculated and the van der Waals interaction, hydrogen bonds and hydrophobic interactions play an important role in stabilizing the complex. Besides, we also studied the effect of Cu²⁺, Ni²⁺, Mn²⁺and Co²⁺ on the binding constants between daidzin and BSA, it is shows that the binding of BSA and daidzin is strengthened in the presence metal ions.

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Mechatronics and Mechanical Engineering I

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Periodical:

Applied Mechanics and Materials (Volume 664)

Pages:

402-409

DOI:

https://doi.org/10.4028/www.scientific.net/AMM.664.402

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Online since:

October 2014

Authors:

Qing Ming Wang, Jia Liu, Tian Xing Zhang, Feng Zhu, Xin Hui Tang

Keywords:

Bovine Serum Albumin (BSA), Daidzin, Fluorescence Quenching, Static Quenching

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© 2014 Trans Tech Publications Ltd. All Rights Reserved

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