Inhibition of TGFα-Saporin on Neointimal Hyperplasia via Inhibiting Smooth Muscle Cells Proliferation and Promoting its Apoptosis

Abstract:

Objective: To investigate the effects of TGFα-SAP on the inhibition of neointimal hyperplasia of rat which suffered from common carotid arterial injury. Methods: Saporin and TGFα were conjugated by using N-succinimidyl-3 (2-pyridyldithio) propionate. seventy rats were divided into two groups at random, the TGFα-SAP treated group was treated with local injection of TGFα-SAP (5μg/kg) after injury, and the control group was treated with saline. PCNA analysis and TUNEL analysis were performed. Results: Compared to the control group, the TGFα-SAP treated group showed a significant inhibition of intimal thickness and exhibited a lower P/A ratio and a higher rate of apoptosis. Conclusion: TGFα-SAP, as a potential stent coating material, could inhibit neointimal hyperplasia by inhibiting the proliferation and promoting the apoptosis of smooth muscle cells in the neointima.

• Periodical: Applied Mechanics and Materials (Volume 665)
• Pages: 323-326
• DOI: https://doi.org/10.4028/www.scientific.net/AMM.665.323
• Online since: October 2014
• Authors: Jian Luo, Ou Zeng, Ting Xiao, Fang Li, Zhi Xiong Wu, Chun Chu, Xi Yong Yu, Shu Guang Lin, Jun Yang
• Keywords: Coating Material, Cytotoxicity, Saporin, Stent, Transforming Growth Factor-α

References

[1] Liao D, Lin PH, Yao Q, Chen C. Vascular smooth cell proliferation in perfusion culture of porcine carotid arteries. Biochemical and biophysical research communications 2008; 372: 668-73.

DOI: 10.1016/j.bbrc.2008.05.117

[2] Horigome M, Kumazaki S, Hattori N, Kasai H, Aizawa K, Izawa A, et al. Noninvasive evaluation of coronary endothelial function following sirolimus-eluting stent implantation by using positron emission tomography. Cardiology 2009; 114: 157-63.

DOI: 10.1159/000226093

[3] Pfisterer M, Brunner-La Rocca HP, Buser PT, Rickenbacher P, Hunziker P, Mueller C, et al. Late clinical events after clopidogrel discontinuation may limit the benefit of drug-eluting stents: an observational study of drug-eluting versus bare-metal stents. Journal of the American College of Cardiology 2006; 48: 2584-91.

DOI: 10.1016/j.jacc.2006.10.026

[4] Polito L, Bortolotti M, Farini V, Battelli MG, Barbieri L, Bolognesi A. Saporin induces multiple death pathways in lymphoma cells with different intensity and timing as compared to ricin. The international journal of biochemistry & cell biology 2009; 41: 1055-61.

DOI: 10.1016/j.biocel.2008.09.021