New Advancement on Cross-Linked Enzyme Aggregates within Magnetically-Separable Mesoporous Silica

Abstract:

Magnetically-separable enzyme system has been developed by adsorption, precipitation and cross-linking of enzymes in superparamagnetic hierarchically ordered mesoporous mesocellular silica (M-HMMS). The immobilization of xylanase within M-HMMS were compared between enzyme adsorption (EA), enzyme adsorption and cross-linking (EAC), and enzyme adsorption, precipitation and cross-linking (EAPC). EAPC includes higher enzyme activity immobilized within the matrix in comparison with the other methods. Furthermore, the immobilized enzyme is predicted to be prevented from leaching out of the matrix when exterior blow is being tested on the structure. Thus, the stability of the EAPC of this invention is anticipated to be maintained even after a long time passed since high enzyme activity compared with known method can be supported and immobilized within the matrix. Consequently, it is possible to improve performance of the enzymes by manipulating the preparation and operation condition.

• Periodical: Applied Mechanics and Materials (Volume 818)
• Pages: 276-280
• DOI: https://doi.org/10.4028/www.scientific.net/AMM.818.276
• Online since: January 2016
• Authors: Nurul Jannah Sulaiman, Roshanida Abd Rahman
• Keywords: Cross-Linked Enzyme Aggregates, Magnet, Mesoporous Silica, Xylanase

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