Influence of Organic Supplements on Growth and Development of In Vitro Shoots of Bulbophyllum dhaninivatii Seidenf

Article Preview

Abstract:

In Vitro shoot culture of Bulbophyllum dhaninivatii Seidenf. was conducted on semi-solid Vacin and Went (1949) medium supplemented with coconut water (50 100 and 150 ml/L), potato extract (25 and 50 g/L) and banana homogenate (25 and 50 g/L) for 12 weeks. The results found that the highest shoot number (6.92 shoots) could observe on the medium supplemented with 150 ml/L coconut water, 50 g/L potato extract and 50 g/L banana homogenate while the highest leaf and root number could obtain when cultured on the medium supplemented with 100 ml/L coconut water, 50 g/L potato extract and 50 g/L banana homogenate.

You have full access to the following eBook

Info:

* - Corresponding Author

[1] E.S. Siegerist, Bulbophyllums and their allies: a grower's guide, Timber Press, Inc., Portland, Oregon, (2001).

Google Scholar

[2] Information on http: /apps. kew. org/wcsp/advsearch. do; jsessionid=123D10881BDE409A 73896025EA7EC668.

Google Scholar

[3] O. Thaithong, Orchids of Thailand, Office of Environmental Policy and Planning, Bangkok, Thailand, (1999).

Google Scholar

[4] M.M. Hossain, M. Sharma, P. Pathak, In vitro propagation of Dendrobium aphyllum (Orchidaceae)-seed germination to flowering. J. Plant Biochem. Biotechnol. 22(2) (2013) 157–167.

DOI: 10.1007/s13562-012-0124-3

Google Scholar

[5] D. Zhao, G. Hu, Z. Chen, Y. Shi, L. Zheng, A. Tang, C. Long, Micropropagation and in vitro flowering of Dendrobium wangliangii: a critically endangered medicinal orchid. J. Med. Plants Res. 7(28) (2013) 2098-2110.

DOI: 10.5897/jmpr11.1777

Google Scholar

[6] S. Parthibhan, M.V. Rao, T.S. Kumar, In vitro regeneration from protocorms in Dendrobium aqueum Lindley – an imperiled orchid. J. Gen. Eng. Biotechnol. 13 (2015) 227-233.

DOI: 10.1016/j.jgeb.2015.07.001

Google Scholar

[7] J.A.T. da Silva, J.C. Cardoso, J. Dobra´nszki, S. Zeng, Dendrobium micropropagation: a review. Plant Cell Rep. 34 (2015) 671-704.

DOI: 10.1007/s00299-015-1754-4

Google Scholar

[8] J.A.T. da Silva, M. Tanaka, Culture vessel affects hybrid Cymbidium protocorm-like body and callus induction. Flor. Ornament. Biotechnol. 3(1) (2009) 53-55.

Google Scholar

[9] J.A.T. da Silva, New basal media for protocorm-like body and callus induction of hybrid Cymbidium. J. Fruit Ornament. Plant Res. 20(2) (2012) 127-133.

DOI: 10.2478/v10290-012-0022-8

Google Scholar

[10] P. Mohanty, S. Paul, M.C. Das, S. Kumaria, P. Tandon, A simple and efficient protocol for the mass propagation of Cymbidium mastersii: an ornamental orchid of Northeast India. AoB PLANTS 2012: pls023; doi: 10. 1093/aobpla/pls023.

DOI: 10.1093/aobpla/pls023

Google Scholar

[11] S.K. Bhadra, H. Barua, M.M. Hossain, In vitro germination and rapid micropropagation of Bulbophyllum lilacinum Redley. Bangladesh J. Bot. 33(2) (2004)103-107.

Google Scholar

[12] Y.I. Lee, E. Yeung, Embryo development and in vitro seed germination of Bulbophyllum fascinator. Acta Hortic. 878 (2010) 243-250.

DOI: 10.17660/actahortic.2010.878.30

Google Scholar

[13] C. Maneerattanarungroj, S. Laywisadkul, A. Kongbangkerd, Tissue culture of Bulbophyllum affine Lindl. NU Sci. J. 7(2) (2011) 45-59.

Google Scholar

[14] M.M.M. Than, In vitro conservation of endangered orchid Bulbophyllum auricomum Lindl. Prop. Ornament. Plants 13(4) (2013) 154-159.

Google Scholar

[15] S.P. Vij, A. Kher, P. Pathak, Regeneration competence of Bulbophyllum careyanum (Hook. ) Spreng. pseudobulb segments. J. Orchid Soc. India 14 (2000) 47–55.

Google Scholar

[16] M.M.M. Than, A. Pal, S. Jha, In vitro flowering and propagation of Bulbophyllum auricomum Lindl., the royal flower of Myanmar. Acta Hortic. 829 (2009) 105-111.

DOI: 10.17660/actahortic.2009.829.14

Google Scholar

[17] M.M.M. Than, A. Pal, S. Jha, Plant regeneration from callus cultures in endangered orchid Bulbophyllum auricomum Lindl. Prop. Ornament. Plants, 12(2) (2012) 102-108.

Google Scholar

[18] E. Vacin, F. Went, Some pH changes in nutrient solutions. Bot. Gaz. 110 (1949) 605–613.

DOI: 10.1086/335561

Google Scholar

[19] S. Aktar, K.M. Nasiruddin, K. Hossain, Effects of different media and organic additives interaction on in vitro regeneration of Dendrobium orchid. J. Agric. Rural Dev. 6(1-2) (2008) 69-74.

DOI: 10.3329/jard.v6i1.1659

Google Scholar

[20] S. Kaur, K.K. Bhutani, Organic growth supplement stimulants for in vitro multiplication of Cymbidium pendulum (Roxb. ) Sw. Hort. Sci. (Prague) 39(1) (2012) 47–52.

DOI: 10.17221/52/2011-hortsci

Google Scholar

[21] K. Obsuwan, C. Thepsithar, An effect of organic supplements on stimulating growth of Vanda and Mokara seedlings in tissue culture. Int. J. Biol. Biomolec. Agric. Food Biotechnol. Eng. 8(7) (2014) 696-698.

Google Scholar

[22] D. David, R. Jawan, H. Marbaw, J.A. Gansau, Organic additives improves the in vitro growth of native orchid Vanda helvola Blume. Not. Sci. Biol. 7(2) (2015) 192-197.

DOI: 10.15835/nsb729546

Google Scholar

[23] Y.S. Huh, J.K. Lee, S.Y. Nam, K.Y. Paek, G.U. Suh, Improvement of asymbiotic seed germination and seedling development of Cypripedium macranthos Sw. with organic additives. J. Plant Biotechnol. 43 (2016) 138-145.

DOI: 10.5010/jpb.2016.43.1.138

Google Scholar

[24] J.W.H. Yong, L. Ge, Y.F. Ng, S.N. Tan, The chemical composition and biological properties of coconut (Cocos nucifera L. ) water. Molecules 2009, 14, 5144-5164; doi: 10. 3390/molecules 14125144.

DOI: 10.3390/molecules14125144

Google Scholar

[25] P. Gnasekaran, R. Poobathy, M. Mahmood, M.R. Samian, S. Sreeramanan, Effect of complex additives on improving the growth of PLBs of Vanda Kasem's Delight. Aus. J. Crop Sci. 6(8) (2012) 1245-1248.

Google Scholar

[26] N. Nambiar, C.S. Tee, M. Maziah, Effects of organic additives and different carbohydrate sources on proliferation of protocorm-like bodies in Dendrobium Alya Pink. Plant Omics J. 5(1) (2012) 10-18.

Google Scholar

[27] M.O. Islam, M. Akter, A.K.M.A. Prodhan, Effect of potato extract on in vitro seed germination and seedling growth of local Vanda roxburgii orchid. J. Bangladesh Agric. Univ. 9(2) (2011) 211–215.

DOI: 10.3329/jbau.v9i2.10988

Google Scholar

[28] Md.A. Baque, Y.K. Shin, T. Elshmari, E.J. Lee, K.P. Paek, Effect of light quality, sucrose and coconut water concentration on the microporpagation of Calanthe hybrids (Bukduseong' × 'Hyesung' and 'Chunkwang' × 'Hyesung, ). Aus. J. Crop Sci. 5(10) (2011).

Google Scholar

[29] P. Sinha, S.K. Roy, Regeneration of an indigenous orchid, Vanda teres (Roxb. ) Lindl. Through in vitro culture. Plant Tiss. Cult. 14 (2004) 55–61.

Google Scholar

[30] M. Minea, C. Piluek, T.A. Menakani, S. Tantiwiwat, A study on seed germination and seedling development of Spathoglottis kimballiana Bl. orchids. Kasetsart J. (Nat. Sci. ) 38 (2004) 141-156.

Google Scholar