The Synthesis of Poly-β-Hydroxybutyrate by Moderately Halophilic Bacteria Halomonas venusta

Shuang Gao; Ling Hua Zhang

doi:10.4028/www.scientific.net/AMR.1033-1034.306

Paper Titles

Synthesis and Biological Activities of 1,2,4-Triazine Derivatives
p.283

Terpenoids from Chloranthus multistachys
p.287

The Antimicrobial Activity of Fructuskochiae Ethanol Extract and its Impact on the Rheological Properties of the Shampoo
p.291

The Study of Senkyunolide I Degradation Products by Ultra-High Performance Liquid Chromatography-Quadrupole Time-of-Flight Mass Spectrometry
p.298

The Synthesis of Poly-β-Hydroxybutyrate by Moderately Halophilic Bacteria Halomonas venusta
p.306

Microwave Assisted Synthesis of Isoquercitrin Using Enzymatic Hydrolysis of Rutin
p.311

Preparation of Compound Danshen Micropowder Tablets and Comparative Study of Pharmacokinetics with Compound Danshen Extraction Tablets
p.315

Study on Synthesis of a Series of Hydroxyl Functional Cholesterylated Hepatic Targeting Liposome Ligands
p.320

Study on Synthesis of a Series of Amino Functional Cholesterylated Hepatic Targeting Liposome Ligands
p.324

HomeAdvanced Materials ResearchAdvanced Materials Research Vols. 1033-1034The Synthesis of Poly-β-Hydroxybutyrate by...

The Synthesis of Poly-β-Hydroxybutyrate by Moderately Halophilic Bacteria Halomonas venusta

Abstract:

In this study, poly-β-hydroxybutyrate (PHB) was synthesized by moderately halophilic bacteria Halomonas venusta and identified by ¹H-NMR. The effects of different carbon sources, salt concentration, initial carbon nitrogen quality ratio, initial phosphate concentration were studied on the synthesis of PHB. The optimum conditions of the PHB synthesis were detemined. Glucose was as carbon source with the concentration of 80 g/L and salt concentration, initial carbon nitrogen ratio, initial phosphate concentration was 3%, 15 and 1.9 g/L respectively. Under the above conditions, a two-phase synthesis system of PHB was constructed. The PHB synthesis amount and yield of cell dry weight was reached up to 1.5 g/L and 69.19 wt.%, respectively. The efficient synthesis of PHB was realized.

You might also be interested in these eBooks

Advances in Chemical Engineering and Advanced Materials IV

View Preview

Info:

Periodical:

Advanced Materials Research (Volumes 1033-1034)

Pages:

306-310

DOI:

https://doi.org/10.4028/www.scientific.net/AMR.1033-1034.306

Citation:

Cite this paper

Online since:

October 2014

Authors:

Shuang Gao*, Ling Hua Zhang

Keywords:

Halomonas venusta, Moderately Halophilic Bacteria, Poly-β-Hydroxybutyrate (PHB)

Export:

RIS, BibTeX

Price:

Permissions CCC:

Request Permissions

Permissions PLS:

Request Permissions

Сopyright:

Citation:

* - Corresponding Author

References

[1] Lee SY. Plastic bacteria. Progress and prospects for polyhydroxyalkanoate production in bacteria. Trends Biotechnol, Vol. 14 (1996), p.431.

DOI: 10.1016/0167-7799(96)10061-5

Google Scholar

[2] Steinbüchel A, Füchtenbush B. Bacterial and other biological systems for polyester production. Trends Biotechnol, Vol. 16 (1998), p.419.

Google Scholar

[3] Ventosa A, Nieto J, Oren A. Biology of moderately halophilic aerobic bacteria. Microbiol Mol Biol Rev, Vol. 62 (1998) p.504.

DOI: 10.1128/mmbr.62.2.504-544.1998

Google Scholar

[4] Oren A. Diversity of halophilic microorganisms: environments, phylogeny, physiology, and applications. J Ind Microbiol Biotechnol, Vol. 28 (2002), p.56.

DOI: 10.1038/sj/jim/7000176

Google Scholar

[5] Margesin R, Schinner F. Potential of halotolerant and halophilic microorganisms for biotechnology. Extremophiles, Vol. 5 (2001) p.73.

DOI: 10.1007/s007920100184

Google Scholar

[6] Quillaguamán J, Delgado O, Mattiasson B, Hatti-Kaul R. Poly(β-hydroxybutyrate) production by a moderate halophile, Halomonas boliviensis LC1. Enzyme Microb Tech, Vol. 38 (2006) p.148.

DOI: 10.1016/j.enzmictec.2005.05.013

Google Scholar

[7] Yilmaz M, Soran H, Beyatli Y. Determination of poly-β-hydroxybutyrate (PHB) production by some Bacillus spp. World J Microb Biot, Vol. 21 (2005) p.565.

DOI: 10.1007/s11274-004-3274-1

Google Scholar

[8] Grasshoff K, Kremling K, Ehrhardt M,. Methods of seawater analysis. Weinheim: WILEY-VCH Verlag GmbH, (1999) p.170.

Google Scholar

[9] Thirumala M, Reddy SV, Mahmood SK. Production and characterization of PHB from two novel strains of Bacillus spp. isolated from soil and activated sludge. J Ind Microbiol Biotechnol, Vol. 37 (2010) p.271.

DOI: 10.1007/s10295-009-0670-4

Google Scholar

[10] Mohamed A. Abdelwahab, ElisaMartinelli, etc. Poly[(R)-3-hydroxybutyrate)]/ Poly(styrene) Blends Compatibilized with the Relevant Block Copolymer. Journal of polymer science, Vol. 50 (2012) p.5151.

DOI: 10.1002/pola.26358

Google Scholar

[11] Zhang LH, Lang YJ, Nagata S. Efficient production of ectoine using ectoine-excreting strain. Extremophiles, Vol. 13 (2009) p.717.

DOI: 10.1007/s00792-009-0262-2

Google Scholar