Optimization of Extracting Condition of Active Components in Perilla by Ultrasonic

Dan Huang; You Qing Liu; Yuan Liang; Xiang Mao

doi:10.4028/www.scientific.net/AMR.1033-1034.713

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Optimization of Extracting Condition of Active Components in Perilla by Ultrasonic

Abstract:

Using ultrasonic-extraction technology, the condition of extracting the active compounds-flavonoids and rosmarinic acid of Perilla was studied. The effects of four main factors include ultrasonic power, temperature, time and solid-liquid ratio on the extraction-rate of the flavonoids and the rosmarinic acid of Perilla were reviewed by single factor experiments,and the flavonoids and the rosmarinic acid was determined by NaNO₂-Al (NO₃)₃-NaOH method and FeSO₄ method. On the base of single factor experiments, the extraction technique was optimized in the orthogonal test. The result showed that temperature was the most important factor in the influencing factors on the extraction-rate. The subsequence of importance was temperature > time > solid-liquid ratio > ultrasonic. The optimal extraction conditions were: extraction solvent 3%borax, solid-liquid ratio 1:50(w/v), ultrasonic power 300W, time 90min , temperature 75°C. Under the optimum technological conditions, 34.64mg/g of flavonoids and rosmarinic acid from Perilla could be obtained.