For Libraries For Publication Open Access Downloads About Us Contact Us
Paper Titles
Optimization of Anthocyanins Extraction from Blueberry Pomace by Spray-Drying Process by Response Surface Methodology
p.709
Optimization of Extracting Condition of Active Components in Perilla by Ultrasonic
p.713
Optimization of Subcritical 1,1,1,2-Tetrafluoroethane( R134a ) Removal of Cholesterol from Spray-Dried Sthenoteuthis oualaniensis Egg Powder Using Response Surface Methodology
p.717
Preparation and Properties of Glycerol Plasticized-Corn Starch/Nanoparticles Bionan°Composites
p.724
Prepared Polyclonal Antibody of the Ciprofloxacin
p.728
Research on Detecting Aluminum in Jellyfish by Flame Atomic Absorption Method
p.732
Research on the Extraction and Purification of Dihydromyricetin from Ampelopsis grossedentata
p.738
Screening of Mold Producing Aroma in Chinese Koji and Optimization of Culture Conditions
p.744
Study on Oxidation Stability of Algae Oil
p.748

Prepared Polyclonal Antibody of the Ciprofloxacin

Article Preview

Abstract:

To detect Ciprofloxacin (CPFX) residues in food stuffs of animal origin rapidly by immune method, preparation of high quality polyclonal antibody against CPFX was the most critical step of project. We produced two kinds of antigens for CPFX, using carrier protein bovine serum albumin (BSA) and ovalbumin (OVA) by a modified carbodiimide method, and then the hapten-protein conjugates were characterized by ultraviolet spectrophotometry in detecting qualitatively comparable hapten density, before being used for the immunization and detection purposes. The production of polyclonal antibodies (PcAbs) were sought following the generation of appropriate CPFX-BSA conjugate. One PcAb against Ciprofloxacin (CPFX) was produced and the titer polyclonal antibody could reach 6.25×105. In the optimized ELISA, the PcAb showed 50% inhibition at 10.93 ng/mL for CPFX in buffer.

You might also be interested in these eBooks
Advances in Chemical Engineering and Advanced Materials IV View Preview

Info:

Periodical:

Advanced Materials Research (Volumes 1033-1034)

Pages:

728-731

DOI:

https://doi.org/10.4028/www.scientific.net/AMR.1033-1034.728

Citation:

Cite this paper

Online since:

October 2014

Authors:

Xuang Yun Huang, Dong Mei Huang, You Qiong Cai*

Keywords:

Ciprofloxacin, Conjuguate, Hapten, Polyclonal Antibody

Export:

RIS, BibTeX

Price:

Permissions CCC:

Request Permissions

Permissions PLS:

Request Permissions

Сopyright:

© 2014 Trans Tech Publications Ltd. All Rights Reserved

Share:

Citation:

* - Corresponding Author

References

[1] M. Hernandez, C. Aguilar and F. Borrull, J. Chromatogr B. Vol. 772(2002), 163–172.

Google Scholar

[2] H.G. Ovando, N. Gorla and C. Luders, J. Vet Pharmacol Ther. Vol. 22(1999), 209-212.

Google Scholar

[3] J.H. Duan and Z.H. Yuan. J. Agric. Food Chem. Vol. 49(2001), 1087-1089.

Google Scholar

[4] M. Fuentes, J .M. Palomo and C. Mateo. J. Immunol. Methods, Vol. 307, 144-149.

Google Scholar

[5] A.F.S. A Habeeb. Arch. Biochem. Biophy. Vol. 121(1967), 652-664.

Google Scholar

[6] S. Pauillac, J. Naar and P. Branaa. J. Immunol. Methods. Vol. 220(1998), 105–114.

Google Scholar

[7] M. P Marco, S. Gee and B. D. Hammock. Trac-trend. Anal. Chem, Vol. 14(1995), 415-425.

Google Scholar

[8] K.V. Singh, J. Kaur and C.V. Grish. Bioconjugate Chem, Vol. 15(2004), 168-173.

Google Scholar

[9] S. Pauillac, J. Naar and M. Barbara. J. Immunol. Methods. 263(2002), 75– 83.

Google Scholar

[10] Y. Zhou, Y.S. Li and Z. Wang. Chin. J. Vet. Sci. Vol. 26(2006), 200-203.

Google Scholar

[11] A.F.S.A. Habeeb. Anal Biochem. Vol. 14(1966), 328-336.

Google Scholar

© 2025 Trans Tech Publications Ltd. All rights are reserved, including those for text and data mining, AI training, and similar technologies. For open access content, terms of the Creative Commons licensing CC-BY are applied.
Scientific.Net is a registered trademark of Trans Tech Publications Ltd.