A Novel hig E Aptamer Biosensor Base on Core-Shell Fe₃O₄@Au Magnetic Composite Nanoparticles

Abstract:

In this paper, a novel hIg E aptamer biosensor was designed base on the core-shell Fe₃O₄@Au magnetic composite nanoparticles (Fe₃O₄@Au NPs). Firstly, Fe₃O₄@Au NPs were prepared by one-step reduction process with Fe₃O₄ nanoparticles (Fe₃O₄ NPs) as magnetic core and hydroxylamine hydrochloride as deoxidizer. Then, the morphology, composition, and properties of Fe₃O₄@Au NPs were characterized by scanning electron microscope (SEM), fourier translation infrared spectroscopy (FT-IR), vibrating sample magnetometer (VSM) and so on. Lastly, Human immunoglobulin E (hIg E) was used as the model analyte, a hIg E aptamer biosensor was presented which the hIg E antibody is covalently immobilized as the capture probe on Fe₃O₄@Au NPs surface, and hIg E aptamer was used as the detection probe. After the hIg E antigen was captured, the ascorbic acid 2-phosphate (AAP) formed ascorbic acid (AA) in the presence of alkaline phosphatase (ALP). The AA reduced the silver ions (Ag⁺) in the solution to silver metal that preferentially deposited on surface of the Fe₃O₄@Au NPs. The amount of deposited silver could be quantified using the electrochemical methods. The oxidation current of Ag⁰ was linear with the concertration of hIg E over the range 0.25~2.0 μg/mL. Therefore, the hIg E aptamer biosensor possessed higher sensitivity, low detection limit and rapid response speed.

• Periodical: Advanced Materials Research (Volume 1118)
• Pages: 170-175
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.1118.170
• Online since: July 2015
• Authors: Guo Yin Huang, Long Fei Ma, Ming Yuan Guan, Jin Tao Liang, Yong Huang*, Gui Yin Li
• Keywords: Aptamer, Biosensor, Fe₃O₄@Au Nanoparticles, Human Immunoglobulin E

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