The Molecular Characterization and Hydrogen Production of a New Species of Anaerobe in Wastewater Treatment Process for H₂ Recovery

Abstract:

The isolation and identification of hydrogen production bacteria(HPB)with high yield and high evolution rate is an important foundation of fermented hydrogen production process by anaerobic digesting high strength organic wastewater. An improved Hungater rolling tubes technique and a plate method of culture bottle (PMCB) were employed to enumerate and isolate the anaerobes. The HPB-RL medium was designed specially for isolating and culturing anaerobic hydrogen bacteria. Culture temperature was at 37°C and 5.5 of pH. About 90 strains of bacteria were isolated, in which, a strain of the hydrogen producer with high yield and high evolution rate was isolated and named Rennanqi12. The isolates were straight rods, Grams positive, strictly anaerobic, no spore-forming bacteria, whole bacteria flagella, having capsule, 3~4 metachromatic granules. The strain of Rennanqi12 could produce hydrogen using molasses wastewater as the carbon resource. The isolate Rennanqi12 has the closest relationship with the strain C. cellulose for the 94% homology of 16S rDNA. The strain of Rennanqi12 might be a new species that belongs to a new genus. The amount of hydrogen-producing (YH2)is 1880.5ml/L medium and the maximum hydrogen-producing rate(QH2)is 28.2 mmol/g•(dry•cell)•h when R12 digested substrates were glucose.

• Periodical: Advanced Materials Research (Volumes 113-116)
• Pages: 1049-1052
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.113-116.1049
• Online since: June 2010
• Authors: Xiao Ye Liu, Zi Rui Guo, Yong Juan Zhang, Zhan Qing Wang, Yong Feng Li
• Keywords: 16S rDNA Sequencing, Anaerobe, H₂-Producing Capacity, Hydrogen Production

References

[1] Hawkes, F. R.; Dionsdale, R.; Hawkes, D.L. and Hussy, I.. Sustainable fermentative hydrogen production: challenges for process optimization. Int. J. Hydrogen Energy. 27(11-12), (2002), pp.1339-1347.

DOI: 10.1016/s0360-3199(02)00090-3

[2] Tanisho, S. and Ishiwata, Y. Continuous hydrogen production from molasses by the bacterium Enterobacter aerogenes. Int. J. Hydrogen Energy. 19(10) (1994), pp.807-812.

DOI: 10.1016/0360-3199(94)90197-x

[3] Li, Y.F.; Ren, N.Q.; Chen, Y., Li J.Z., Hu, L.J.; and Zheng. G.X. The experimental Anaerobic Operation of Isolation and Culture on Hydrogen-producing and Fermentative Bacteria. Journal of Harbin Institute of technology. 36(12) (2004).

[4] Holt, J. G.; Krieg, N. R.; Sneath, P. H. A.; Staley, J. T.; Williams, S. T. Bergey's manual of determinative bacteriology, 9th ed.; Williams & Wilkins: Baltimore, Md., U.S.A. (1994).

[5] Li, Y.F., Ren, N.Q., Hu, L.J., Zheng, G.X., Li, J. Z., Chen, Y., Lin, H. L, and Zhang, W.Q. Characterization and the sequence analysis of 16S rDNA of a new species of high efficient biohydrogen production anaerobe. Journal of Harbin Institute of technology. 37(3) (2005).

[6] Li Y.F., Ren N.Q., Yang C.P., Li J. Z., Zheng G.X. DESAR for energy recovery: hydrogen production process for a new H2-producing anaerobe from the activated sludge. In Proceedings of the International Water Association (IWA) Conference 2005 on Future of Urban Wastewater System-Decentralisation and Reuse (Wang, X.C. and Chen, R. Eds. ), Xi'an, China, 18-20 May (2005).

[7] Ren, N.Q.; Wang, B.Z.; Huang, J.L. Ethanol-type fermentation from carbohydrate in high rate acidogenic reactor. Biotechnol. Bioeng. 54(5) (1997), pp.428-433.

DOI: 10.1002/(sici)1097-0290(19970605)54:5<428::aid-bit3>3.0.co;2-g

[8] Fry, N.K.; Warwick, S. and Saunders, N. A. The use 16S ribosomal RNA analyses to investigate phylogeny of the family Legionellaceae. J. Gen. Microbiol. 137 (1991), p.12151222.

DOI: 10.1099/00221287-137-5-1215