Laccase Immobilization on D380 Macroporous Exchange Resin by Cross-Linking with Glutaraldehyde

Jie Zhang; Yang Yu; Jing Ren; Bin Song Wang

doi:10.4028/www.scientific.net/AMR.113-116.2115

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HomeAdvanced Materials ResearchAdvanced Materials Research Vols. 113-116Laccase Immobilization on D380 Macroporous...

Laccase Immobilization on D380 Macroporous Exchange Resin by Cross-Linking with Glutaraldehyde

Abstract:

Macroporous exchange resins have been applied as supports for enzyme immobilization. The supporters were activated by cross-linking with glutaraldehyde, then Laccase was immobilized. Parameters of the immobilization process were optimized. The results of repeated batch showed the optimal immobilization conditions (10% of glutaraldehyde, pH=3, temperature=45 °C). In addition, the biocatalyst also showed good operational stability, maintaining 45% of its initial activity after 10 cycles of oxidation of ABTS. The immobilized laccase had potential for application in the continuous decolorization of textile efﬂuents.

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Periodical:

Advanced Materials Research (Volumes 113-116)

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2115-2118

DOI:

https://doi.org/10.4028/www.scientific.net/AMR.113-116.2115

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Online since:

June 2010

Authors:

Jie Zhang, Yang Yu, Jing Ren, Bin Song Wang

Keywords:

Cross-Linking, Immobilisation, Laccase, Macroporous Resins

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