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The Ability of Indigenous Bacterial Isolates and their Biosurfactants to Treat the Oily Sludge in the South Refineries Company, Basrah, Iraq

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Abstract:

The oily sludge at the South Refineries firm in Basrah Governorate, Southern Iraq, was treated with biosurfactant made from native bacterial isolates. Consequently, 33 bacterial isolates were obtained from 11 samples of oily sludge, water, and soil obtained from various locations within the organization. These isolates were identified biochemically using the VITEK II system and genetically using 16sRNA. The bacterial species identified by the findings were Bacillus cereus, Aeromonas hydrophila/caviae, Staphylococcus lentus, and S. pseudointermedius. The biosurfactant was manufactured by selecting one isolate from each species. The bacteria and their biosurfactants showed a remarkable ability to break down oily sludge. Bacillus cereus was able to reduce 99.16% of the aliphatic fraction, and Staphylococcus lentus 99.69% of the aromatic fraction. These findings suggest that these bacteria could be utilized for bioremediation of environments polluted by petrochemical oily sludge, resulting in reduced pollution and increased industrial output.

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Periodical:

Advanced Materials Research (Volume 1186)

Pages:

191-199

DOI:

https://doi.org/10.4028/p-My2wTf

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Online since:

January 2026

Authors:

Eman A. Al-Imara, Zaki A. Madan, Amjed Alabresm*, S.M. Salih, Sabah M. Al-Shatty, Ahmed A. Al-Salhi

Keywords:

Aliphatic, Bacteria, Genetic Identification, Hydrocarbons, Oily Sludge, Sustainable Development

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© 2026 Trans Tech Publications Ltd. All Rights Reserved

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