A Novel Amperometric Immunosensor Based on Thionine/DNA Self-Assembled Multilayers on Carbon Nanotubes Modified Glass Carbon Electrode

Abstract:

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A novel amperometric immunosensor for the determination of alpha-fetoprotein (AFP) was constructed using multi-wall carbon nanotubes modified glass carbon electrode.thionine as the mediator,were immobilized by DNA throngh the layer-by-layer method. Thionine was first fabricated on carbon nanotubes modified glass carbon throngh 1-Ethyl-3-(3-dimethylaminopropyl)carbodiimide/N-hydroxysuccinimide(EDC/NHS).then a negatively charged DNA film was absorbed on the positively charged thionine,and the DNA was uesd as cross-linker to immobiled amount thionine.nano-Au-was used to immobilized anti-AFP.The electrochemical sensor have a relatively low detection limit of 0.02ng/ml.

Info:

Periodical:

Advanced Materials Research (Volumes 160-162)

Edited by:

Guojun Zhang and Jessica Xu

Pages:

1170-1175

DOI:

10.4028/www.scientific.net/AMR.160-162.1170

Citation:

L. Y. Jia et al., "A Novel Amperometric Immunosensor Based on Thionine/DNA Self-Assembled Multilayers on Carbon Nanotubes Modified Glass Carbon Electrode", Advanced Materials Research, Vols. 160-162, pp. 1170-1175, 2011

Online since:

November 2010

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$35.00

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