A Novel Amperometric Immunosensor Based on Thionine/DNA Self-Assembled Multilayers on Carbon Nanotubes Modified Glass Carbon Electrode

Abstract:

A novel amperometric immunosensor for the determination of alpha-fetoprotein (AFP) was constructed using multi-wall carbon nanotubes modified glass carbon electrode.thionine as the mediator,were immobilized by DNA throngh the layer-by-layer method. Thionine was first fabricated on carbon nanotubes modified glass carbon throngh 1-Ethyl-3-(3-dimethylaminopropyl)carbodiimide/N-hydroxysuccinimide(EDC/NHS).then a negatively charged DNA film was absorbed on the positively charged thionine,and the DNA was uesd as cross-linker to immobiled amount thionine.nano-Au-was used to immobilized anti-AFP.The electrochemical sensor have a relatively low detection limit of 0.02ng/ml.

• Periodical: Advanced Materials Research (Volumes 160-162)
• Pages: 1170-1175
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.160-162.1170
• Online since: November 2010
• Authors: Li Yong Jia, Ning Gan, Lei Zheng, Qian Wang
• Keywords: Carbon Nanotube (CN), DNA, GNP, Layer-By-Layer, Thionine

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