Delamination is one of the important damage modes in the fiber-reinforced composite laminates. The interlaminar fracture toughness is the key parameter in delamination failure analysis of composites. The stress analysis by a finite element modeling has shown that the shear stress is very large near the ply splicing area. So the delamination failure is mainly dependent on the mode II fracture toughness. A new way of loading in tensile testing is proposed for the measurement of mode II fracture toughness. Specific specimen with splicing plies has been designed and used for the experiment. Testing study on the carbon-fiber-reinforced laminate with ply splicing was performed. Steady crack propagation has been seen by the tensile testing of the specimen. Mode II fracture toughness GIIC of the laminate has been determined by the experiment data analysis. The experiment and numerical analysis shows that the tensile testing for the measurement of mode II interlaminar fracture toughness is feasible.