A novel polyethersulfone (PES) tubular membrane preparation by phase inversion via immersion precipitation using N,N-dimethylacetamide (DMAc) as the solvent, PEG-400 as the additive. The effects of membrane preparation conditioans on membrane performance were investigated. It was found that the flux was increased by adding ethanol into the coagulation bath. And the low temperature of coagulation bath also improve membrane’s rejection. Test results show that the flux can be up to 160 L•m-2h-1 and the rejection can reach more than 97% (for ovalbvmin) under optimized conditions.