Purification and Characterization of Chitinases from Bacillus licheniformis Using Auricularia auricular Colloidal Substance as a Substrate
A chitinase was purified from the culture supernatant of Bacillus licheniformis with Auricularia auricular colloidal substance as the sole carbon source. The optimized conditions of this species strain for the production of chitinases were found to be when the culture was shaken at 37°C for 3 days in medium (pH 7) containing 0.05% KH2PO4, 0.5%NaNO3, 0.05% MgSO4·7H2O and 1.5% Auricularia auricular colloidal substance powder(w/v). The molecular weights of the chitinases determined by SDS-PAGE were approximately 52kDa. The chitinases was inhibited highly by Mg2+ and PMSF, whereas Co2+ and Mn2+ could increase highly the activity of the chitinase.
Zhong Cao, Lixian Sun, Xueqiang Cao, Yinghe He
Y. J. Zhang et al., "Purification and Characterization of Chitinases from Bacillus licheniformis Using Auricularia auricular Colloidal Substance as a Substrate", Advanced Materials Research, Vols. 233-235, pp. 1014-1017, 2011