Purification and Characterization of Chitinases from Bacillus licheniformis Using Auricularia auricular Colloidal Substance as a Substrate

Abstract:

A chitinase was purified from the culture supernatant of Bacillus licheniformis with Auricularia auricular colloidal substance as the sole carbon source. The optimized conditions of this species strain for the production of chitinases were found to be when the culture was shaken at 37°C for 3 days in medium (pH 7) containing 0.05% KH₂PO₄, 0.5%NaNO₃, 0.05% MgSO₄·7H₂O and 1.5% Auricularia auricular colloidal substance powder(w/v). The molecular weights of the chitinases determined by SDS-PAGE were approximately 52kDa. The chitinases was inhibited highly by Mg²⁺ and PMSF, whereas Co²⁺ and Mn²⁺ could increase highly the activity of the chitinase.

• Periodical: Advanced Materials Research (Volumes 233-235)
• Pages: 1014-1017
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.233-235.1014
• Online since: May 2011
• Authors: Yong Jun Zhang, Qiao Xian, Jie Min He, Meng Yao Zhao
• Keywords: Auricularia auricular, Bacillus licheniformis, Chitinase

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