Extraction, Purification and Antioxidant Activity of Intracellular Polysaccharides from a Strain of Lachnum sp.
The microwave method was used to extract Lachnum calyculiforme polysaccharides (LCP). By orthogonal experiments, the optimal conditions of extraction were the microwave temperature was 120 °C, extraction time was 20 min, ratio of water to raw material was 60:1, and the yield of polysaccharides was 12.56 %. LCP-1 was separated and sequentially purified from LCP through Sephadex G-100 column chromatography, which was detected as the homogeneous component. LCP -1 had strong scavenging abilities on O2ˉ•, •OH and DPPH free radicals.
Zhong Cao, Yinghe He, Lixian Sun and Xueqiang Cao
M. Ye et al., "Extraction, Purification and Antioxidant Activity of Intracellular Polysaccharides from a Strain of Lachnum sp.", Advanced Materials Research, Vols. 236-238, pp. 2114-2117, 2011