Extraction, Purification and Antioxidant Activity of Intracellular Polysaccharides from a Strain of Lachnum sp.

Ming Ye; Ru Yue Yuan; Tao Qiu; Jing Min Cai

doi:10.4028/www.scientific.net/AMR.236-238.2114

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HomeAdvanced Materials ResearchAdvanced Materials Research Vols. 236-238Extraction, Purification and Antioxidant Activity...

Extraction, Purification and Antioxidant Activity of Intracellular Polysaccharides from a Strain of Lachnum sp.

Abstract:

The microwave method was used to extract Lachnum calyculiforme polysaccharides (LCP). By orthogonal experiments, the optimal conditions of extraction were the microwave temperature was 120 °C, extraction time was 20 min, ratio of water to raw material was 60:1, and the yield of polysaccharides was 12.56 %. LCP-1 was separated and sequentially purified from LCP through Sephadex G-100 column chromatography, which was detected as the homogeneous component. LCP -1 had strong scavenging abilities on O2ˉ•, •OH and DPPH free radicals.