Extraction and Separation of Angiotensin-Converting Enzyme Inhibitor from Enzymatic Hydrolysate of Oyster

Ru Qiang Huang; Niang Hui Li; Qing Zhu Zeng; Qian Deng; Bin Chen

doi:10.4028/www.scientific.net/AMR.236-238.2610

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HomeAdvanced Materials ResearchAdvanced Materials Research Vols. 236-238Extraction and Separation of...

Extraction and Separation of Angiotensin-Converting Enzyme Inhibitor from Enzymatic Hydrolysate of Oyster

Abstract:

The oyster shell was a common sort of marine shellfish, and its meat and shell serve as traditional medicinal materials. To invest the method in which ACEI (Angiotensin-Converting Enzyme Inhibitor) can be prepared, this experiment took oyster as study objective, employed inhibitory coefficient as standard and utilized tryptase as an enzyme. Moreover, this study carried out the chromatogram separation of the hydrolysate by using Sephadex G-25, and measured inhibition coefficient as well as relative molecular weight distribution of ACEI from hydrolysate. The result showed that after the chromatogram separation, the highest activity was determined at the second elution peak at which the active coefficient of ACEI was 89.51%, and that the relative molecular weight distributed between 1842Da and 1396Da.

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Periodical:

Advanced Materials Research (Volumes 236-238)

Pages:

2610-2614

DOI:

https://doi.org/10.4028/www.scientific.net/AMR.236-238.2610

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Online since:

May 2011

Authors:

Ru Qiang Huang, Niang Hui Li, Qing Zhu Zeng, Qian Deng, Bin Chen

Keywords:

ACEI, Hydrolysate, Oyster

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References

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