Study In Situ on Rat Intestinal Absorption Mechanism and Influential Factors of Ginsenosides Rb1 and Rg1 in Bazhen Decoction

Abstract:

To investigate the absorption of ginsenosides Rg₁ and Rb₁ in Bazhen Decoction at different intestine segments of rats and the influence of the drug solution concentration, pH, P-gp inhibitor on ones. The intestine cannulation was performed for in situ recirculation. The concentration of ginsenosides Rg₁ and Rb₁, and phenol red in the flux were measured by the reversed phase HPLC and ultraviolet spectrophotometry, respectively. The absorption of ginsenosides Rg₁ and Rb₁ in Bazhen Decoction in intestine of rat show first-order kinetics with the absorption mechanism of passive diffusion. Ginsenoside Rg₁ has specific absorption locus of duodenum and ginsenoside Rb₁ has specific absorption locus of jejunum. Meanwhile, ginsenoside Rb₁ is the P-gp substrate, and could increase its fraction of bioavailability by corporation with P-gp inhibitor.

• Periodical: Advanced Materials Research (Volumes 282-283)
• Pages: 457-460
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.282-283.457
• Online since: July 2011
• Authors: Wen Lan Li, Jing Xin Ding, Jing Bai, Li Lli Nan, Yang Yang, Yu Bin Ji
• Keywords: Bazhen Decoction, Ginsenoside Rb₁, Ginsenoside Rg₁, High Performance Liquid Chromatography (HPLC), In Situ Intestinal Absorption

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