Microstructures of electron beam welded joints for TA15 titanium alloy with different hydrogen contents were observed and analyzed by SEM and TEM. And the influence of hydrogen on microstructure of the joints was investigated. The results show that the microstructure of the weld metal is lamellar α+β phase after hydrogen charging. In the range of hydrogen contents discussed in this study (from 0 to 0.101 wt%), With the increase of hydrogen content, there is little change in the appearance of the microstructure of the weld metal. The presence of hydrogen can promote the growth of twins in electron beam welded joints. With the increase of hydrogen content, the number of twins is increased. When hydrogen content reaches to a certainty level, hydrides are found in TA15 electron beam welded joints.