Preparation of Nicotinamide Adenine Dinucleotide Functionalized Multi-Walled Carbon Nanotube and its Application to Dehydrogenase Biosensor
Coenzyme, nicotinamide adenine dinucle-otide (NAD+) functionalized multi-walled carbon-nanotubes (MWCNTs) were prepared in this work. Carboxylic groups were firstly introduced to MWCNTs by oxidation in a mixture of sulfuric acid and nitric acid, and the NAD moistures were further attached by the formation of amide bonds with carboxylic groups under the help of 1-ethyl-3-(3-dimethyl-aminopropyl)carbodiimidehydrochloride and N-hydroxysuccinimide, which resulted in the coenzyme functionalized materials, MWCNTs-NAD. The MWCNTs-NAD was characterized by UV-Vis, FT-IR and TEM, and it was found clearly that the MWCNTs-NAD exhibited coenzyme activity compared with native NAD+ in reaction catalyzed by NAD-dependent alcohol dehydrogenase. The application of MWCNTs-NAD on the construction of dehydrogenase biosensor was further investigated.
Tianbiao Zhang and Jerry Tian
X. S. Wang et al., "Preparation of Nicotinamide Adenine Dinucleotide Functionalized Multi-Walled Carbon Nanotube and its Application to Dehydrogenase Biosensor", Advanced Materials Research, Vol. 298, pp. 121-127, 2011