Preparation of Nicotinamide Adenine Dinucleotide Functionalized Multi-Walled Carbon Nanotube and its Application to Dehydrogenase Biosensor

Xing Sheng Wang; Pu Liu; Hai Tao Zheng; Hui Hu; Wen Juan Zheng; Shinichiro Suye

doi:10.4028/www.scientific.net/AMR.298.121

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HomeAdvanced Materials ResearchAdvanced Materials Research Vol. 298Preparation of Nicotinamide Adenine Dinucleotide...

Preparation of Nicotinamide Adenine Dinucleotide Functionalized Multi-Walled Carbon Nanotube and its Application to Dehydrogenase Biosensor

Abstract:

Coenzyme, nicotinamide adenine dinucle-otide (NAD+) functionalized multi-walled carbon-nanotubes (MWCNTs) were prepared in this work. Carboxylic groups were firstly introduced to MWCNTs by oxidation in a mixture of sulfuric acid and nitric acid, and the NAD moistures were further attached by the formation of amide bonds with carboxylic groups under the help of 1-ethyl-3-(3-dimethyl-aminopropyl)carbodiimidehydrochloride and N-hydroxysuccinimide, which resulted in the coenzyme functionalized materials, MWCNTs-NAD. The MWCNTs-NAD was characterized by UV-Vis, FT-IR and TEM, and it was found clearly that the MWCNTs-NAD exhibited coenzyme activity compared with native NAD+ in reaction catalyzed by NAD-dependent alcohol dehydrogenase. The application of MWCNTs-NAD on the construction of dehydrogenase biosensor was further investigated.

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Advanced Materials Research (Volume 298)

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121-127

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https://doi.org/10.4028/www.scientific.net/AMR.298.121

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Online since:

July 2011

Authors:

Xing Sheng Wang, Pu Liu, Hai Tao Zheng, Hui Hu, Wen Juan Zheng, Shinichiro Suye

Keywords:

Biosensor, Coenzyme, Multi-Walled Carbon Nanotube (MWCNT), NAD-Dependent Dehydrogenase

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