DNA Electrochemical Biosensor Based on Chitosan and Gold Nanoparticles Using Methylene Blue as Electrochemical Indicator

Ai Chun Zhang; Cun Zhou

doi:10.4028/www.scientific.net/AMR.298.128

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Preparation of Nicotinamide Adenine Dinucleotide Functionalized Multi-Walled Carbon Nanotube and its Application to Dehydrogenase Biosensor
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DNA Electrochemical Biosensor Based on Chitosan and Gold Nanoparticles Using Methylene Blue as Electrochemical Indicator
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HomeAdvanced Materials ResearchAdvanced Materials Research Vol. 298DNA Electrochemical Biosensor Based on Chitosan...

DNA Electrochemical Biosensor Based on Chitosan and Gold Nanoparticles Using Methylene Blue as Electrochemical Indicator

Abstract:

In this paper, a novel DNA electrochemical biosensor based on layer-by-layer self-assembled technology was reported. Gold nanoparticles were firstly immobilized on chitosan modified gold electrode by large amino of chitosan because of strong electrostatic adsorption effect between amino and gold atom, and thoil group modified DNA (SH-ssDNA) probe sequence was then self-assembled onto the electrode. Cyclic voltammetry (CV) and differential pulse voltammetry (DPV) were applied to investigate the electrochemical behavior of different modified electrodes with methylene blue as hybrid indicator. The results in optimization experiment condition show that: The peak current difference value pre and post hybridization was linearly related to the logatithmic value of the target DNA concentration ranging from 10-8~10-5mol/L.A detection limit of 3.55×10-9mol/L can be estimated.

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Periodical:

Advanced Materials Research (Volume 298)

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128-134

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https://doi.org/10.4028/www.scientific.net/AMR.298.128

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Online since:

July 2011

Authors:

Ai Chun Zhang, Cun Zhou

Keywords:

Chitosan (CS), DNA Electrochemical Biosensor, Gold Nanoparticles (GNP), Methylene Blue (MB)

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References

[1] F. Li, W. Chen, S. S. Zhang. Development of DNA electrochemical biosensor based on covalent immobilization of probe DNA by direct coupling of sol-gel and self-assembly technologies[J]. Biosensors and Bioelectronics, 2008, 24: 781-786.

DOI: 10.1016/j.bios.2008.06.052

Google Scholar

[2] N. L. Rosi, C. A. Mirkin, Nanostructures in biodiagnostics[J], Chem. Rev, 2005, 105: 1547-1562.

DOI: 10.1021/cr030067f

Google Scholar

[3] T. G. Drummond, M. G. Hill, J. K. Barton, Electrochemical DNA sensors [J]. Nat. Biotechnol, 2003, 21: 1192-1199.

DOI: 10.1038/nbt873

Google Scholar

[4] L. He, M. D. Musick, S. T. Nicewarner, F. G. Salinas, S. J. Benkovic, M. J. Natan,C. D. Keating, Colloidal Au-enhanced surface Plasmon resonance for ultrasensitive detection of DNA hybridization[J], Am. Chem. Soc. 2000, 122: 9071-9077.

DOI: 10.1021/ja001215b

Google Scholar

[5] D. Ozkan, P. Kara, K. Kerman, etc. DNA and PNA sensing on mercury and carbon electrodes by using methylene blue as an electrochemical label[J]. Bioelectrochemistry, 2002, 58: 119-126.

DOI: 10.1016/s1567-5394(02)00131-7

Google Scholar

[6] F. Lucarelli., G. Marrazza, A. P. F. Turner., et al., Carbon and gold electrodes as electrochemical transducers for DNA hybridisation sensors, Biosens. Bioelectron., 2004, 19: 515~530.

DOI: 10.1016/s0956-5663(03)00256-2

Google Scholar

[7] P. Du, H. X. Li, Z. H. Mei, etc. Electrochemical DNA biosensor for the detection of DNA hybridization with the amplification of Au nanoparticles and CdS nanoparticles[J]. Bioelectrochemistry, 2009, 75: 37-43.

DOI: 10.1016/j.bioelechem.2009.01.003

Google Scholar

[8] K. Ito, K. Hashimoto, I Y. shimoti. et al. Anal. Chim. Acta, 1996, 327: 29-35.

Google Scholar

[9] Z. H. Feng, Medical molecular biology[M]People health press, 2003: 245.

Google Scholar