Cotton fabric was treated by chitosan and epoxy-silicone finishing agents to improve its wrinkle-resistant property. The study showed that mixed finish by two agents can provide higher wrinkle-resistance than single finish when applied to the cotton fabrics due to their synergistic effect. In the wrinkle-resistant finishing process, four main factors (chitosan mass concentration, organosilicon volume concentration, baking temperature and baking time) influencing finish effect were discussed and then process conditions were determined. After optimum process treatment, the wrinkle-recovery angle of mixed finished cotton fabric increased evidently while its whiteness and breaking strength dropped a little. In addition, the addition of organosilicon can improve fabric softness. After washed for 30 times, delay wrinkle-recovery angle of mixed finished fabric was 54° higher than that of native fabric, and the treated cotton exhibited good washing resistance.