Effects of Long-Term Elevated CO₂ on Rhizosphere and Bulk Soil Bacterial Community Structure in Pinus sylvestriformis Seedlings Fields

Abstract:

The effect of long-term elevated CO₂ (as open top chambers) on rhizosphere and bulk bacterial community structure in Pinus sylvestriformis seedlings field was investigated in July, August, and September. The bacterial communities were processed by Denaturing Gradient Gel Electrophoresis (DGGE) analysis of bacterial 16S rDNA fragments amplified by PCR (Polymerase Chain Reaction) from DNA extracted directly from soil. DGGE profiles from rhizosphere samples showed large changes in rhizosphere bacterial community under elevated CO₂ compared to ambient except for that in September. For bulk samples, bacterial community structure changed when exposed to elevated CO₂ in three months. With the exception of bulk samples in August, a similitude of bacterial communities structures existed between different elevated CO₂ concentrations by analyzing UPGMA dendrogram based on Jaccard’s coefficient.

• Periodical: Advanced Materials Research (Volumes 343-344)
• Pages: 351-356
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.343-344.351
• Online since: September 2011
• Authors: Xia Jia, Chun Juan Zhou
• Keywords: Bacterial Community, DGGE, Effect, Elevated CO₂

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