This study was conducted to evaluate the performance of membrane bioreactors (MBR) for organics and nitrogen removal. The membrane bioreactor was fed with domestic sewage and operated at different contents of dissolved oxygen (DO), different hydraulic retention time (HRT), and various mixed liquor suspended solid (MLSS) concentrations. The results showed that the distribution of DO level in MBR imposed a significant effect on simultaneous nitrification–denitrification (SND), and the optimal DO concentration should be controlled between 0.5 mg/L to1 mg/L. The denitrification was found to show the best performance for total nitrogen (TN) removal when the HRT reached 5 hours. Higher MLSS concentration led to the improvement in TN removal and the optimal MLSS concentration was 9000mg/L. In fact, more than 90% Chemical Oxygen Demand (COD) and ammonia nitrogen (NH3-N) were reduced when the MLSS concentration exceeded 3000mg/L.