Synthesis and Identification of Norfloxacin Artificial Antigen

Abstract:

A multiresidue immunoassay method for determination of Fluoroquinolones (FQs) residues has been developed. For this purpose, NHS ester technology was employed to synthesize the immunogen and coating antigen of Norfloxacin (NFLX). SDS-PAGE, UV-visible spectra and Infrared spectra identification showed that the artificial antigen was conjugated successfully. Based on the square matrix titration, an icELISA method was established. The dynamic range in assay buffer was from 0.038 to 112.8 ng/mL, with LOD and IC50 value of 0.02 ng/mL and 1.2 ng/mL, respectively. This assay showed a high cross-reactivity to Ciprofloxacin (86%), Enrofloxacin (75%), Difloxacin (63%), Sarafloxacin (57%) and Pefloxacin (33.8%). The chemical effects on assay performance showed that the physiological pH (7.4) in assay buffer pursued the maximum absorbance (A_max) and the most sensitive IC50 values. The results suggest the artificial antigen was synthesized successfully, and the established immunoassay could be used for simultaneous detecting of Norfloxacin, Ciprofloxacin, Enrofloxacin, Difloxacin, Sarafloxacin and Pefloxacin residues in animal-original food samples.

• Periodical: Advanced Materials Research (Volumes 433-440)
• Pages: 697-702
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.433-440.697
• Online since: January 2012
• Authors: Jun Wei Liu, Jin Qing Jiang, Hai Tang Zhang, Guo Ying Fan, Zhi Xing An
• Keywords: Artificial Antigen, Fluoroquinolones (FQs), Indirect Competitive ELISA, Norfloxacin, Polyclonal Antibody

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