Study on the Isolation of High-Quality DNA and RNA from Aloe

Abstract:

. Aloe, an important folk herbal drug, includes abundant polysaccharides and secondary metabolites which bringing about the difficulty of isolating high-quality DNA or RNA. In this paper, one and two improved methods were used to isolate the genomic DNA and RNA from the leaf of Aloe, respectively. The obtained samples presented good quality and integrality, and thus, they could be further used for many downstream molecular experiments. This reported protocols on extraction of DNA and RNA offered a valuable reference for other related studies.

• Periodical: Advanced Materials Research (Volumes 455-456)
• Pages: 449-454
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.455-456.449
• Online since: January 2012
• Authors: Yong Si Zhang, Jun Li, Xiao Hong Liu
• Keywords: Aloe, DNA, Extraction, Purification, RNA

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