Preparation and Characterization of Immobilization of Lipase in Amino-Functionalized Mesoporous Silica

Chun Feng Wang; Guo Wei Zhou; Lei Zhang

doi:10.4028/www.scientific.net/AMR.486.187

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Preparation and Characterization of Immobilization of Lipase in Amino-Functionalized Mesoporous Silica

Abstract:

Porcine pancreatic lipase (PPL) was immobilized in ordered mesoporous silica materials, which were using PEG 2000 (poly (ethylene glycol)) and P123 as dual templates and synthesized via hydrothermal process. The materials were silylated by (3aminopropyl) triethoxysilane (APTES) by postsynthesis-grafting method. Samples were characterized by XRD, TEM, and BET. The results showed that the 2-dimensional hexagonal mesoscopic structure kept unchanging after modification. As compared to lipase immobilized mesoporous silica, the loading amount and hydrolization activity of lipase immobilized on aminopropyl-grafted mesoporous silica increased, respectively.

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Periodical:

Advanced Materials Research (Volume 486)

Pages:

187-192

DOI:

https://doi.org/10.4028/www.scientific.net/AMR.486.187

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Online since:

March 2012

Authors:

Chun Feng Wang, Guo Wei Zhou, Lei Zhang

Keywords:

(3-aminopropyl)triethoxysilane (APTES), Immobilisation, Lipase, Mesoporous Silica, Poly(ethylene glycol) (PEG)

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