Studies on Immobilization of Penicillin G Acylase to Epoxy Resin

Abstract:

The process conditions of immobilizing penicillin G acylase(PGA) by epoxy resin were studied. This experiment used the Box-Behnken experimental design and response surface methodology(RSM) to optimize the conditions of immobilizing PGA by epoxy resin. The results showed the best process conditions were pH 8.1, temperature 29°C, carrier of epoxy resin 1g and reaction time 24 h. On these conditions, the activity of the immobilized enzyme was 365.76 U g^-1, activity recovery rate was 62.82%. The characteristics of the immobilized PGA under optimal conditions had been measured and found that the optimum pH of immobilized enzyme was 9.0, the optimum temperature was 60°C. It has better continuous operation stability.

• Periodical: Advanced Materials Research (Volumes 512-515)
• Pages: 1699-1711
• DOI: https://doi.org/10.4028/www.scientific.net/AMR.512-515.1699
• Online since: May 2012
• Authors: Chao Fan, Ji Lie Li, Xiao Yuan Zhu, Nan Li, Wei Wang, Lu Guo, Qian Luo
• Keywords: Box-Behnken Experimental Design, Epoxy Resin (ER), Immobilisation, PGA, Response Surface Methodology (RSM)

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